真核表达survivin基因对曲古菌素A诱导卵巢癌细胞凋亡作用的影响
目的探讨survivin基因对曲古菌素A（TSA）诱导卵巢癌细胞凋亡作用的影响。方法（1）将本实验室已构建成功的survivin正义全长真核表达质粒（pcDNA3．1-survivin），经脂质体包裹转染人卵巢癌细胞A2780，以转染pcDNA3．1空载体的A2780细胞为对照。（2）RT-PCR和Western blot方法分别检测survivin mRNA和蛋白质的表达。（3）MTT比色法和流式细胞仪（FACS）分别检测TSA对两组细胞存活率和凋亡率的影响。（4）Western blot检测TSA作用下A2780细胞中survivin蛋白的表达变化。结果（1）RT-PCR和Western...
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| Published in | Zhongliu fangzhi yanjiu Vol. 35; no. 9; pp. 609 - 612 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
华中科技大学同济医学院附属同济医院妇产科,武汉,430030
2008
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-8578 |
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| Abstract | 目的探讨survivin基因对曲古菌素A（TSA）诱导卵巢癌细胞凋亡作用的影响。方法（1）将本实验室已构建成功的survivin正义全长真核表达质粒（pcDNA3．1-survivin），经脂质体包裹转染人卵巢癌细胞A2780，以转染pcDNA3．1空载体的A2780细胞为对照。（2）RT-PCR和Western blot方法分别检测survivin mRNA和蛋白质的表达。（3）MTT比色法和流式细胞仪（FACS）分别检测TSA对两组细胞存活率和凋亡率的影响。（4）Western blot检测TSA作用下A2780细胞中survivin蛋白的表达变化。结果（1）RT-PCR和Western blot检测提示转染pcDNA3．1-survivin组中survivin mRNA和蛋白质表达明显高于空载体组。（2）MTT比色法和FACS检测提示转染pcDNA3．1-survivin组细胞存活率明显高于空载体组，细胞凋亡率明显低于空载体组，差异有统计学意义（P〈0．05）。pcDNA3．1-survivin转染后的A2780细胞对TSA的敏感性明显降低。（3）Westernblot检测提示survivin的表达水平随着TSA作用时间的延长而下降。结论曲古菌素A诱导卵巢癌细胞的凋亡作用可能与survivin基因表达有关。 |
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| AbstractList | R737.31; 目的 探讨survivin基因对曲古菌素A(TSA)诱导卵巢癌细胞凋亡作用的影响.方法 (1)将本实验室已构建成功的survivin正义全长真核表达质粒(PcDNA3.1-survivin),经脂质体包裹转染人卵巢癌细胞A2780,以转染pcDNA3.1空栽体的A2780细胞为对照.(2)RT-PCR和Western blot方法分别检测survivin mRNA和蛋白质的表达.(3)MIT比色法和流式细胞仪(FACS)分别检测TSA对两组细胞存活率和凋亡率的影响.(4)Western blot检测TsA作用下A2780细胞中survivin蛋白的表达变化.结果 (1)RT-PCR和Western blot检测提示转染pcDNA3.1-survivin组中survivin mRNA和蛋白质表达明显高于空载体组.(2)MTT比色法和FACS检测提示转染PCDNA 3.1-survivin组细胞存活率明显高于空载体组,细胞凋亡率明显低于空载体组,差异有统计学意义(P<0.05).PcDNA3.1-survivin转染后的A2780细胞对TSA的敏感性明显降低.(3)Western blot检测提示survivin的表达水平随着TSA作用时间的延长而下降.结论 曲古菌素A诱导卵巢癌细胞的凋亡作用可能与survivin基因表达有关. 目的探讨survivin基因对曲古菌素A（TSA）诱导卵巢癌细胞凋亡作用的影响。方法（1）将本实验室已构建成功的survivin正义全长真核表达质粒（pcDNA3．1-survivin），经脂质体包裹转染人卵巢癌细胞A2780，以转染pcDNA3．1空载体的A2780细胞为对照。（2）RT-PCR和Western blot方法分别检测survivin mRNA和蛋白质的表达。（3）MTT比色法和流式细胞仪（FACS）分别检测TSA对两组细胞存活率和凋亡率的影响。（4）Western blot检测TSA作用下A2780细胞中survivin蛋白的表达变化。结果（1）RT-PCR和Western blot检测提示转染pcDNA3．1-survivin组中survivin mRNA和蛋白质表达明显高于空载体组。（2）MTT比色法和FACS检测提示转染pcDNA3．1-survivin组细胞存活率明显高于空载体组，细胞凋亡率明显低于空载体组，差异有统计学意义（P〈0．05）。pcDNA3．1-survivin转染后的A2780细胞对TSA的敏感性明显降低。（3）Westernblot检测提示survivin的表达水平随着TSA作用时间的延长而下降。结论曲古菌素A诱导卵巢癌细胞的凋亡作用可能与survivin基因表达有关。 |
| Author | 马晓黎 邢辉 吴鹏 翁丹卉 卢运萍 马丁 |
| AuthorAffiliation | 华中科技大学同济医学院附属同济医院妇产科,武汉430030 |
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| Snippet | 目的探讨survivin基因对曲古菌素A（TSA）诱导卵巢癌细胞凋亡作用的影响。方法（1）将本实验室已构建成功的survivin正义全长真核表达质粒（pcDNA3．1-survivin），经脂质体包裹转染人卵巢癌细胞A2780，以转染pcDNA3．1空载体的A2780细胞为对照。（2）RT-PCR和Western... R737.31; 目的 探讨survivin基因对曲古菌素A(TSA)诱导卵巢癌细胞凋亡作用的影响.方法... |
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| SubjectTerms | survivin基因 卵巢肿瘤 曲古菌素A 细胞凋亡 |
| Title | 真核表达survivin基因对曲古菌素A诱导卵巢癌细胞凋亡作用的影响 |
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