广西百色烟区烟草青枯病菌的遗传多样性分析
【目的】分析广西百色烟区烟草青枯病菌的遗传多样性,为了解该烟区青枯病的发生流行规律和该病原菌的致病机制提供参考。【方法】通过致病力测定、生化型鉴定及BOX-PCR对69株来源于广西烟草主产区百色烟区的青枯菌株的遗传多样性进行系统分析。【结果】供试菌株存在明显的致病力分化,强致病力菌株、中等致病力菌株和弱致病力菌株出现频率分别为17.39%、62.32%和20.29%,其中以中等致病力菌群为优势菌群;供试菌株的生化型复杂,其中37株属于生化型III或生化亚型III-1、III-3和III-4,23株属于生化型Ⅰ,1株属于生化型Ⅱ,另有8株属于非标准生化型;BOX-PCR分析结果表明百色烟草青枯病...
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Published in | 南方农业学报 Vol. 48; no. 5; pp. 825 - 830 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
广西农业科学院 微生物研究所,南宁,530007%广西壮族自治区烟草公司 百色市公司,广西 百色,533000%广西农业科学院 微生物研究所, 南宁 530007
2017
广西大学 农学院, 南宁 530004 |
Subjects | |
Online Access | Get full text |
ISSN | 2095-1191 |
DOI | 10.3969/j.issn.2095-1191.2017.05.011 |
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Summary: | 【目的】分析广西百色烟区烟草青枯病菌的遗传多样性,为了解该烟区青枯病的发生流行规律和该病原菌的致病机制提供参考。【方法】通过致病力测定、生化型鉴定及BOX-PCR对69株来源于广西烟草主产区百色烟区的青枯菌株的遗传多样性进行系统分析。【结果】供试菌株存在明显的致病力分化,强致病力菌株、中等致病力菌株和弱致病力菌株出现频率分别为17.39%、62.32%和20.29%,其中以中等致病力菌群为优势菌群;供试菌株的生化型复杂,其中37株属于生化型III或生化亚型III-1、III-3和III-4,23株属于生化型Ⅰ,1株属于生化型Ⅱ,另有8株属于非标准生化型;BOX-PCR分析结果表明百色烟草青枯病菌存在丰富的遗传多样性,聚类分析结果显示菌株的遗传多态性与菌株的地理来源、致病力、生化型具有一定的相关性,但并无明显的地理种群、生化型种群或致病力一致的种群聚在一起。【结论】广西百色烟区烟草青枯病菌具有丰富的遗传多样性和复杂的生化型,可能是该烟区烟草青枯病为害逐年加重的重要原因之一。 |
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Bibliography: | 45-1381/S tobacco; Ralstonia solanacearum ; biovar; genetic diversity; Baise, Guangxi Objective】Genetic diversity analysis for Ralstonia solanacearum isolated from tobacco in Baise, Guangxi was studied in order to explore occurrence and epidemic regulation in Baise tobacco planting region and pathogenic mechanism of R. solanacearum. 【Method】Sixty-nine representative strains of R. solanacearum isolated from different places in Baise tobacco planting area of Guangxi were analyzed for genetic diversity by pathogenicity and biovar determination, and Box-PCR analysis. 【Result】All the tested strains presented obvious pathogenicity differentiation. The proportions belonging to high, moderate and low pathogenicity were 17.39%, 62.32% and 20.29% respectively. Strains with moderate pathogenicity were dominant ones. Base on biovar test, the biovar of the tested strains were complex. Thirty-seven strains belonged to biotype Ⅲ or subtype biochemical Ⅲ-1, Ⅲ-3 and Ⅲ-4; twenty-three were biotype Ⅰ; one belonged to biotype Ⅱ an |
ISSN: | 2095-1191 |
DOI: | 10.3969/j.issn.2095-1191.2017.05.011 |