蚕沙纤维素降解菌HB-2菌株的分离、鉴定及酶活性分析

【目的】筛选蚕沙纤维素降解菌株,为蚕沙无害化快速腐熟处理提供优质菌种。【方法】以蚕沙为材料,利用CMC-Na培养基分离蚕沙纤维素降解菌,通过形态学、生化特征和16S rDNA序列测定方法等对分离获得的纤维素降解菌进行分类学鉴定,并研究碳源、氮源、pH、培养时间和培养温度对分离菌株产酶活力的影响。【结果】从蚕沙中筛选出1株高温型细菌,命名为HB-2菌株。根据菌体大小、鞭毛着生位置及数量、16S rDNA核酸序列分析,并结合生理生化特性,HB-2菌株鉴定为短小芽孢杆菌（Bacilluspumilus）。HB-2菌株最佳产酶条件为复合碳源（蚕沙＋微晶纤维素＋米糠＋麸皮）、复合氮源（蛋白胨＋酵母膏）,...

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Published in南方农业学报 Vol. 47; no. 12; pp. 2065 - 2071
Main Author 岑贞陆 胡钧铭 韦仕岩 何铁光 李婷婷 曾泉
Format Journal Article
LanguageChinese
Published 广西农业科学院微生物研究所,南宁,530007%广西农业科学院农业资源与环境研究所,南宁,530007 2016
Subjects
短小芽孢杆菌
纤维素降解菌
蚕沙
酶活性
鉴定
identification
蚕沙
鉴定
enzyme activity
cellulose-degrading bacteria
Bacillus pumilus
纤维素降解菌
短小芽孢杆菌
酶活性
silkworm excrement
Online AccessGet full text
ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2016.12.2065

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Summary:【目的】筛选蚕沙纤维素降解菌株,为蚕沙无害化快速腐熟处理提供优质菌种。【方法】以蚕沙为材料,利用CMC-Na培养基分离蚕沙纤维素降解菌,通过形态学、生化特征和16S rDNA序列测定方法等对分离获得的纤维素降解菌进行分类学鉴定,并研究碳源、氮源、pH、培养时间和培养温度对分离菌株产酶活力的影响。【结果】从蚕沙中筛选出1株高温型细菌,命名为HB-2菌株。根据菌体大小、鞭毛着生位置及数量、16S rDNA核酸序列分析,并结合生理生化特性,HB-2菌株鉴定为短小芽孢杆菌（Bacilluspumilus）。HB-2菌株最佳产酶条件为复合碳源（蚕沙＋微晶纤维素＋米糠＋麸皮）、复合氮源（蛋白胨＋酵母膏）,产纤维素酶最适反应pH 6.5,最适温度35℃。【结论】HB-2菌株CMCase活性较敏感且产酶量大,具有制成菌剂应用于蚕沙无害化快速腐熟的潜力。
Bibliography:45-1381/S
silkworm excrement; cellulose-degrading bacteria; Bacillus pumilus; identification; enzyme activity
Objective】 Cellulose-degrading bacteria were isolated from silkworm,in order to provide high-quality strain for harmless quick composting of silkworm excrement.【Method】 Using CMC-Na culture medium,cellulose-degrading bacteria were isolated from silkworm excrement.Then obtained cellulose-degrading bacteria were identified based on its morphology,biochemical characters,16 S rDNA sequence etc.And effects of carbon source,nitrogen source,pH,culture time and culture temperature on cellulase activity of isolated strain were studied.【Result】Results showed that,a strain of high-temperature resistant bacterium was screened out,which was named HB-2 strain.According to its size,flagella position and number,16 S rDNA sequence and physio-biochemical characteristics,HB-2 strain was identified as Bacillus pumilus.Enzyme-producing conditions of HB-2 strain were as follows：composite carbon sources including silkworm exc
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2016.12.2065