黄瓜绿斑驳花叶病毒抗血清制备及应用
用葫芦[Lagenaria siceraria（Molina）Stand．]作为黄瓜绿斑驳花叶病毒（Cucumber green mottle mosaic virus，CGMMV）的繁殖寄主，通过差速离心和PEG二次沉淀法进行病毒提纯，用提纯病毒液免疫新西兰兔制备CGMMV抗血清。制备的CGMMV抗血清经间接ELISA法测定效价为1：5120；利用制备的抗血清检测田间样品，证明其可以用于CGMMV的血清学及免疫捕获RT—PCR检测。研究结果对今后开展葫芦科作物CGMMV检测，及时发现疫情以便采取防控措施，避免病毒扩散为害，确保葫芦科作物的安全生产具有重要意义。...
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| Published in | Guangxi nong ye ke xue Vol. 41; no. 2; pp. 130 - 132 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西农业科学院植物保护研究所,南宁,530007%广西大学农学院,南宁,530005
2010
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-8161 |
| DOI | 10.3969/j.issn.2095-1191.2010.02.011 |
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| Abstract | 用葫芦[Lagenaria siceraria（Molina）Stand．]作为黄瓜绿斑驳花叶病毒（Cucumber green mottle mosaic virus，CGMMV）的繁殖寄主，通过差速离心和PEG二次沉淀法进行病毒提纯，用提纯病毒液免疫新西兰兔制备CGMMV抗血清。制备的CGMMV抗血清经间接ELISA法测定效价为1：5120；利用制备的抗血清检测田间样品，证明其可以用于CGMMV的血清学及免疫捕获RT—PCR检测。研究结果对今后开展葫芦科作物CGMMV检测，及时发现疫情以便采取防控措施，避免病毒扩散为害，确保葫芦科作物的安全生产具有重要意义。 |
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| AbstractList | S436.421.1+9; 用葫芦[Lagenaria siceraria(Molina)Stand.]作为黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)的繁殖寄主,通过差速离心和PEG二次沉淀法进行病毒提纯,用提纯病毒液免疫新西兰兔制备CGMMV抗血清.制备的CGMMV抗血清经间接ELISA法测定效价为1:5120;利用制备的抗血清检测田间样品,证明其可以用于CGMMV的血清学及免疫捕获RT-PCR检测.研究结果对今后开展葫芦科作物CGMMV检测,及时发现疫情以便采取防控措施,避免病毒扩散为害,确保葫芦科作物的安全生产具有重要意义. 用葫芦[Lagenaria siceraria（Molina）Stand．]作为黄瓜绿斑驳花叶病毒（Cucumber green mottle mosaic virus，CGMMV）的繁殖寄主，通过差速离心和PEG二次沉淀法进行病毒提纯，用提纯病毒液免疫新西兰兔制备CGMMV抗血清。制备的CGMMV抗血清经间接ELISA法测定效价为1：5120；利用制备的抗血清检测田间样品，证明其可以用于CGMMV的血清学及免疫捕获RT—PCR检测。研究结果对今后开展葫芦科作物CGMMV检测，及时发现疫情以便采取防控措施，避免病毒扩散为害，确保葫芦科作物的安全生产具有重要意义。 |
| Author | 秦碧霞 蔡健和 黄金玲 胡冬梅 陆秀红 刘志明 |
| AuthorAffiliation | 广西农业科学院植物保护研究所,南宁530007 广西大学农学院,南宁530005 |
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| Author_FL | HUANG Jin-ling CAI Jian-he QIN Bi-xia LU Xiu-hong HU Dong-mei LIU Zhi-ming |
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| Notes | cucumber green mottle mosaic virus indirect ELISA 45-1129/S polyclonal antiserum S436.421.19 PCR cucumber green mottle mosaic virus; polyclonal antiserum; indirect ELISA; PCR |
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| Snippet | 用葫芦[Lagenaria siceraria（Molina）Stand．]作为黄瓜绿斑驳花叶病毒（Cucumber green mottle mosaic... S436.421.1+9; 用葫芦[Lagenaria siceraria(Molina)Stand.]作为黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic... |
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| SubjectTerms | PCR 抗血清 间接ELISA 黄瓜绿斑驳花叶病毒 |
| Title | 黄瓜绿斑驳花叶病毒抗血清制备及应用 |
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