Evaluation of Recombinant Type-Specific Antigens of Orientia tsutsugamushi Expressed by a Baculovirus-Insect Cell System as Antigens for Indirect Immunofluorescence Assay in the Serological Diagnosis of Scrub Typhus

• Published in: Japanese Journal of Infectious Diseases Vol. 73; no. 5; pp. 330 - 335
• Main Authors: Ogawa, Motohiko, Ando, Shuji, Saijo, Masayuki
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 30.09.2020
• Subjects: Animals; Antibodies, Bacterial - blood; Antigens, Bacterial - blood; Baculoviridae - metabolism; Fluorescent Antibody Technique, Indirect - methods; Humans; Immunoglobulin G - blood; Immunoglobulin M - blood; Japan; Mice; Orientia tsutsugamushi; Orientia tsutsugamushi - immunology; Orientia tsutsugamushi - isolation & purification; Recombinant Proteins - immunology; recombinant type-specific antigens; scrub typhus; Scrub Typhus - blood; Scrub Typhus - diagnosis; Serogroup; Serologic Tests; serological diagnosis; Sf9 Cells; Japan; Orientia tsutsugamushi; serological diagnosis; recombinant type-specific antigens; scrub typhus

Scrub typhus (ST) is a mite-borne rickettsiosis caused by the intracellular bacterium Orientia tsutsugamushi (OTS), which is classified as a biosafety level-3 (BSL-3) pathogen. For serological tests of ST, mouse fibroblast cells infected with the five prevalent serotypes of OTS in Japan are generall...