Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids
•LA or OA high-producing strains were obtained by molecular breeding of M. alpina.•The production of LA and OA reached 3.32 and 3.85g/L, respectively, on the 5th day.•The Δ6II RNA level in the transformant was 8 times as high as the wild strain. The aim of this work was to study the molecular breedi...
Saved in:
| Published in | Bioresource technology Vol. 245; no. Pt B; pp. 1610 - 1615 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Ltd
01.12.2017
Elsevier |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | •LA or OA high-producing strains were obtained by molecular breeding of M. alpina.•The production of LA and OA reached 3.32 and 3.85g/L, respectively, on the 5th day.•The Δ6II RNA level in the transformant was 8 times as high as the wild strain.
The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain. |
|---|---|
| AbstractList | The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain. •LA or OA high-producing strains were obtained by molecular breeding of M. alpina.•The production of LA and OA reached 3.32 and 3.85g/L, respectively, on the 5th day.•The Δ6II RNA level in the transformant was 8 times as high as the wild strain. The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain. |
| Author | Kishino, Shigenobu Shima, Jun Sakamoto, Takaiku Ogawa, Jun Bamba, Takeshi Okuda, Tomoyo Sakuradani, Eiji Kikukawa, Hiroshi Izumi, Yoshihiro Ando, Akinori |
| Author_xml | – sequence: 1 givenname: Takaiku surname: Sakamoto fullname: Sakamoto, Takaiku organization: Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, Japan – sequence: 2 givenname: Eiji surname: Sakuradani fullname: Sakuradani, Eiji email: sakuradani.eiji@tokushima-u.ac.jp organization: Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, Japan – sequence: 3 givenname: Tomoyo surname: Okuda fullname: Okuda, Tomoyo organization: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan – sequence: 4 givenname: Hiroshi surname: Kikukawa fullname: Kikukawa, Hiroshi organization: Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan – sequence: 5 givenname: Akinori surname: Ando fullname: Ando, Akinori organization: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan – sequence: 6 givenname: Shigenobu surname: Kishino fullname: Kishino, Shigenobu organization: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan – sequence: 7 givenname: Yoshihiro surname: Izumi fullname: Izumi, Yoshihiro organization: Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan – sequence: 8 givenname: Takeshi surname: Bamba fullname: Bamba, Takeshi organization: Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan – sequence: 9 givenname: Jun surname: Shima fullname: Shima, Jun organization: Faculty of Agriculture, Ryukoku University, 67 Tsukamoto-cho, Fukakusa Fushimi-ku, Kyoto 612-8577, Japan – sequence: 10 givenname: Jun surname: Ogawa fullname: Ogawa, Jun organization: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28673516$$D View this record in MEDLINE/PubMed https://www.osti.gov/biblio/2396013$$D View this record in Osti.gov |
| BookMark | eNqFUU1v3CAURFGqZpP2L0Sop17s8rEGc2sVtUmkRL20Z4ThscvKCxuwK_XfF8ubXHt6As2bmTdzjS5jioDQLSUtJVR8ObRDSHkCu28ZobIloiW9ukAb2kveMCXFJdoQJUjTd2x7ha5LORBCOJXsPbpivZC8o2KDjs8wmSGNwWKIuxABcog7nDxOI5j6keaC_Rx3dTxXwQAZxtFgM55CNNinjPdht8ennNxsp5DiebcSmujwWBnWhw2ufEDvvBkLfDzPG_T7x_dfdw_N08_7x7tvT43tFJkaM_DeDYL2IKxTgzIgGTcdd8p7kPUeJbfWwNBR5jsuiOXeEyK3jCjPOuD8Bn1aeVOZgi42LEHZFCPYSTNeY6EL6PMKqt5fZiiTPoZil-si1Ks1VbTrZd8pWqFihdqcSsng9SmHo8l_NSV66UMf9GsfeulDE6FrH3Xx9qwxD0dwb2uvBVTA1xUANY4_Nd3FLUQLLuTFrEvhfxr_AMTUors |
| CitedBy_id | crossref_primary_10_18016_ksutarimdoga_vi_657322 crossref_primary_10_1016_j_biortech_2019_121672 crossref_primary_10_1016_j_jare_2018_02_003 crossref_primary_10_1007_s00253_020_10958_5 crossref_primary_10_1007_s00253_021_11480_y crossref_primary_10_5650_jos_ess22308 crossref_primary_10_1016_j_bej_2021_108306 crossref_primary_10_1016_j_biortech_2020_124612 crossref_primary_10_1016_j_algal_2022_102861 crossref_primary_10_1016_j_enzmictec_2020_109563 crossref_primary_10_1016_j_jbiosc_2021_02_002 crossref_primary_10_1016_j_jbiosc_2022_02_001 crossref_primary_10_1016_j_biori_2019_02_002 crossref_primary_10_3390_microorganisms11051141 crossref_primary_10_1021_acssynbio_2c00305 crossref_primary_10_1016_j_biotechadv_2023_108298 crossref_primary_10_1016_j_coisb_2019_02_001 crossref_primary_10_1007_s13399_021_02114_4 crossref_primary_10_1021_acssynbio_1c00613 |
| Cites_doi | 10.1128/AEM.00648-09 10.1271/bbb.100001 10.1111/jam.13088 10.1271/bbb.69.1021 10.1016/j.jff.2014.10.007 10.1146/annurev.nutr.22.021302.121842 10.1016/j.jbiosc.2015.01.014 10.1016/S0378-1119(99)00359-5 10.1074/jbc.M605158200 10.1016/j.febslet.2006.12.031 10.1007/s00294-014-0423-4 10.1007/s00253-005-0115-6 10.1128/AEM.71.9.5124-5128.2005 10.1016/j.jff.2015.03.050 10.2174/138920106779116900 10.1074/jbc.M111.266114 10.1001/archinte.160.6.837 10.1021/ie071345u 10.1021/jf049894n 10.1007/BF02932833 10.1016/j.jbiosc.2008.10.011 10.1007/s00253-009-2076-7 10.1073/pnas.92.15.6743 10.1007/s00253-004-1760-x 10.1104/pp.108.117168 10.1006/abio.1995.1419 10.1016/0005-2760(95)00122-S 10.1007/s00253-002-1128-z 10.1016/j.jbiosc.2013.05.023 10.1080/14786419.2014.971316 10.1271/bbb.67.704 10.1007/s10068-013-0116-7 10.1128/AEM.67.3.1246-1252.2001 10.1007/s00253-012-4193-y 10.1007/s12010-016-2182-9 10.1007/s00253-008-1675-z 10.1016/j.jbiosc.2013.04.008 |
| ContentType | Journal Article |
| Copyright | 2017 Elsevier Ltd Copyright © 2017 Elsevier Ltd. All rights reserved. |
| Copyright_xml | – notice: 2017 Elsevier Ltd – notice: Copyright © 2017 Elsevier Ltd. All rights reserved. |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 OTOTI |
| DOI | 10.1016/j.biortech.2017.06.089 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic OSTI.GOV |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering Chemistry Agriculture |
| EISSN | 1873-2976 |
| EndPage | 1615 |
| ExternalDocumentID | 2396013 10_1016_j_biortech_2017_06_089 28673516 S0960852417309938 |
| Genre | Journal Article |
| GroupedDBID | --- --K --M .~1 0R~ 1B1 1RT 1~. 1~5 23N 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM 9JN AAAJQ AABNK AACTN AAEDT AAEDW AAHBH AAHCO AAIKJ AAKOC AALCJ AALRI AAOAW AAQFI AAQXK AARJD AARKO AATLK AAXUO ABFNM ABFYP ABGRD ABGSF ABJNI ABLST ABMAC ABNUV ABUDA ABXDB ACDAQ ACGFS ACIUM ACRLP ADBBV ADEWK ADEZE ADMUD ADQTV ADUVX AEBSH AEHWI AEKER AENEX AEQOU AFKWA AFTJW AFXIZ AGEKW AGHFR AGRDE AGUBO AGYEJ AHEUO AHHHB AHIDL AHPOS AI. AIEXJ AIKHN AITUG AJOXV AKIFW AKRWK AKURH ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BELTK BKOJK BLECG BLXMC CJTIS CS3 DU5 EBS EFJIC EJD ENUVR EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HLV HMC HVGLF HZ~ IHE J1W JARJE KCYFY KOM LUGTX LW9 LY6 LY9 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 PC. Q38 R2- RIG ROL RPZ SAB SAC SDF SDG SDP SEN SES SEW SPC SPCBC SSA SSG SSI SSJ SSR SSU SSZ T5K VH1 WUQ Y6R ~02 ~G- ~KM AAXKI AFJKZ CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 AAIAV OTOTI |
| ID | FETCH-LOGICAL-c590t-ab38db618e6cd9b9ae723a53d9ffe7524974caeb512f5360c3ff0074209f25e33 |
| IEDL.DBID | AIKHN |
| ISSN | 0960-8524 |
| IngestDate | Mon Jul 15 06:08:51 EDT 2024 Fri Oct 25 09:54:35 EDT 2024 Thu Sep 26 21:11:23 EDT 2024 Sat Sep 28 08:49:42 EDT 2024 Sat Aug 10 15:32:01 EDT 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | Pt B |
| Keywords | Mortierella alpina RNAi Fatty acid desaturase Oleic acid Linoleic acid |
| Language | English |
| License | Copyright © 2017 Elsevier Ltd. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c590t-ab38db618e6cd9b9ae723a53d9ffe7524974caeb512f5360c3ff0074209f25e33 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 USDOE Office of Electricity (OE), Advanced Grid Research & Development. Power Systems Engineering Research |
| OpenAccessLink | https://repo.lib.tokushima-u.ac.jp/113896 |
| PMID | 28673516 |
| PQID | 1915878591 |
| PQPubID | 23479 |
| PageCount | 6 |
| ParticipantIDs | osti_scitechconnect_2396013 proquest_miscellaneous_1915878591 crossref_primary_10_1016_j_biortech_2017_06_089 pubmed_primary_28673516 elsevier_sciencedirect_doi_10_1016_j_biortech_2017_06_089 |
| PublicationCentury | 2000 |
| PublicationDate | 2017-12-01 |
| PublicationDateYYYYMMDD | 2017-12-01 |
| PublicationDate_xml | – month: 12 year: 2017 text: 2017-12-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England – name: United Kingdom |
| PublicationTitle | Bioresource technology |
| PublicationTitleAlternate | Bioresour Technol |
| PublicationYear | 2017 |
| Publisher | Elsevier Ltd Elsevier |
| Publisher_xml | – name: Elsevier Ltd – name: Elsevier |
| References | Sakuradani, Murata, Kanamaru, Shimizu (b0130) 2008; 81 Ando, Sumida, Negoro, Suroto, Ogawa, Sakuradani, Shimizu (b0015) 2009; 75 Menendez, Lupu (b0075) 2006; 7 Azcan, Demirel (b0020) 2008; 47 Belury (b0030) 2002; 22 Tamano, Bruno, Karagiosis, Culley, Deng, Collett, Umemura, Koike, Baker, Machida (b0155) 2013; 97 Yamada, Shimizu, Shinmen (b0170) 1987; 51 Kikukawa, Sakuradani, Kishino, Park, Ando, Shima, Ochiai, Shimizu, Ogawa (b0055) 2013; 116 Sakuradani, Ando, Ogawa, Shimizu (b0110) 2009; 84 Sayanova, Haslam, Guschina, Lloyd, Christie, Harwood, Napier (b0140) 2006; 281 Zhang, Sakuradani, Ito, Shimizu (b0185) 2007; 581 Bergamo, Luongo, Miyamoto, Cocca, Kishino, Ogawa, Tanabe, Rossi (b0035) 2014; 11 Sakuradani, Abe, Matsumura, Tomi, Shimizu (b0105) 2009; 107 Wang, Zhang, Pötter, Sun, Li, Yang, Jiao, Zhao (b0165) 2016; 180 Sakuradani, Kamada, Hirano, Nishihara, Kawashima, Akimoto, Higashiyama, Ogawa, Shimizu (b0120) 2002; 60 Takeuchi, Kishino, Park, Hirata, Kitamura, Saika, Ogawa (b0150) 2016; 120 Sakuradani, Ando, Shimizu, Ogawa (b0115) 2013; 116 Sakuradani (b0095) 2010; 74 van de Loo, Broun, Turner, Somerville (b0160) 1995; 18 Takeno, Sakuradani, Tomi, Inohara-Ochiai, Kawashima, Ashikari, Shimizu (b0145) 2005; 71 Sakuradani, Kobayashi, Shimizu (b0125) 1999; 238 Beltrán, Del-Rio, Sánchez, Martínez (b0025) 2004; 52 Sakuradani, Abe, Iguchi, Shimizu (b0100) 2005; 66 Zhou, Green, Singh (b0190) 2011; 286 Ogawa, Matsumura, Kishino, Omura, Shimizu (b0080) 2001; 67 Okuda, Ando, Negoro, Kikukawa, Sakamoto, Sakuradani, Shimizu, Ogawa (b0090) 2015; 120 Okuda, Ando, Sakuradani, Kikukawa, Kamada, Ochiai, Shima, Ogawa (b0085) 2014; 60 Meesapyodsuk, Qiu (b0070) 2008; 147 Higashiyama, Fujikawa, Park, Shimizu (b0045) 2002; 7 Kawashima, Akimoto, Fujita, Naoki, Konishi, Shimizu (b0050) 1995; 229 Sakuradani, Shimizu (b0135) 2003; 67 Ferrara, Raimondi, d'Episcopo, Guida, Dello-Russo, Marotta (b0040) 2000; 160 Kurushima, Hayashi, Shingu, Kuga, Ohtani, Okura, Tanaka, Yasunobu, Nomura, Kajiyama (b0060) 1995; 1258 Yan, Zhuo, Mulan, Xia, Yangmin, Yinbo, Fenghong (b0180) 2013; 22 Matthaus, Özcan, Al-Juhaimi (b0065) 2015; 29 Abe, Sakuradani, Asano, Kanamaru, Ioka, Shimizu (b0005) 2005; 69 Yang, Chen, Stanton, Ross, Zhang, Chen, Chen (b0175) 2015; 15 Abe, Sakuradani, Asano, Kanamaru, Shimizu (b0010) 2006; 70 Ogawa (10.1016/j.biortech.2017.06.089_b0080) 2001; 67 Sakuradani (10.1016/j.biortech.2017.06.089_b0110) 2009; 84 Zhang (10.1016/j.biortech.2017.06.089_b0185) 2007; 581 Belury (10.1016/j.biortech.2017.06.089_b0030) 2002; 22 Bergamo (10.1016/j.biortech.2017.06.089_b0035) 2014; 11 Yamada (10.1016/j.biortech.2017.06.089_b0170) 1987; 51 Sakuradani (10.1016/j.biortech.2017.06.089_b0135) 2003; 67 Sakuradani (10.1016/j.biortech.2017.06.089_b0095) 2010; 74 Kikukawa (10.1016/j.biortech.2017.06.089_b0055) 2013; 116 Sakuradani (10.1016/j.biortech.2017.06.089_b0100) 2005; 66 Menendez (10.1016/j.biortech.2017.06.089_b0075) 2006; 7 Ando (10.1016/j.biortech.2017.06.089_b0015) 2009; 75 Sakuradani (10.1016/j.biortech.2017.06.089_b0105) 2009; 107 Matthaus (10.1016/j.biortech.2017.06.089_b0065) 2015; 29 Sakuradani (10.1016/j.biortech.2017.06.089_b0125) 1999; 238 Wang (10.1016/j.biortech.2017.06.089_b0165) 2016; 180 Sayanova (10.1016/j.biortech.2017.06.089_b0140) 2006; 281 Ferrara (10.1016/j.biortech.2017.06.089_b0040) 2000; 160 Sakuradani (10.1016/j.biortech.2017.06.089_b0115) 2013; 116 Azcan (10.1016/j.biortech.2017.06.089_b0020) 2008; 47 Beltrán (10.1016/j.biortech.2017.06.089_b0025) 2004; 52 Yang (10.1016/j.biortech.2017.06.089_b0175) 2015; 15 Okuda (10.1016/j.biortech.2017.06.089_b0085) 2014; 60 Sakuradani (10.1016/j.biortech.2017.06.089_b0130) 2008; 81 Meesapyodsuk (10.1016/j.biortech.2017.06.089_b0070) 2008; 147 Okuda (10.1016/j.biortech.2017.06.089_b0090) 2015; 120 Takeno (10.1016/j.biortech.2017.06.089_b0145) 2005; 71 Abe (10.1016/j.biortech.2017.06.089_b0005) 2005; 69 van de Loo (10.1016/j.biortech.2017.06.089_b0160) 1995; 18 Kurushima (10.1016/j.biortech.2017.06.089_b0060) 1995; 1258 Takeuchi (10.1016/j.biortech.2017.06.089_b0150) 2016; 120 Abe (10.1016/j.biortech.2017.06.089_b0010) 2006; 70 Higashiyama (10.1016/j.biortech.2017.06.089_b0045) 2002; 7 Sakuradani (10.1016/j.biortech.2017.06.089_b0120) 2002; 60 Tamano (10.1016/j.biortech.2017.06.089_b0155) 2013; 97 Yan (10.1016/j.biortech.2017.06.089_b0180) 2013; 22 Zhou (10.1016/j.biortech.2017.06.089_b0190) 2011; 286 Kawashima (10.1016/j.biortech.2017.06.089_b0050) 1995; 229 |
| References_xml | – volume: 11 start-page: 192 year: 2014 end-page: 202 ident: b0035 article-title: Immunomodulatory activity of a gut microbial metabolite of dietary linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, associated with improved antioxidant/detoxifying defences publication-title: J. Funct. Foods contributor: fullname: Rossi – volume: 147 start-page: 1325 year: 2008 end-page: 1333 ident: b0070 article-title: An oleate hydroxylase from the fungus publication-title: Plant Physiol. contributor: fullname: Qiu – volume: 81 start-page: 497 year: 2008 end-page: 503 ident: b0130 article-title: Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S–4 publication-title: Appl. Microbiol. Biotechnol. contributor: fullname: Shimizu – volume: 75 start-page: 5529 year: 2009 end-page: 5535 ident: b0015 article-title: Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S–4, and its application for eicosapentaenoic acid producer breeding publication-title: Appl. Environ. Microbiol. contributor: fullname: Shimizu – volume: 71 start-page: 5124 year: 2005 end-page: 5128 ident: b0145 article-title: Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S–4, through RNA interference with Δ12-desaturase gene expression publication-title: Appl. Environ. Microbiol. contributor: fullname: Shimizu – volume: 67 start-page: 704 year: 2003 end-page: 711 ident: b0135 article-title: Gene cloning and functional analysis of a second Δ6-fatty acid desaturase from an arachidonic acid-producing publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Shimizu – volume: 120 start-page: 1282 year: 2016 end-page: 1288 ident: b0150 article-title: Efficient enzymatic production of hydroxy fatty acids by linoleic acid Δ9 hydratase from publication-title: J. Appl. Microbiol. contributor: fullname: Ogawa – volume: 15 start-page: 314 year: 2015 end-page: 325 ident: b0175 article-title: Review of the roles of conjugated linoleic acid in health and disease publication-title: J. Funct. Foods contributor: fullname: Chen – volume: 18 start-page: 6743 year: 1995 end-page: 6747 ident: b0160 article-title: An oleate 12-hydroxylase from publication-title: Proc. Natl. Acad. Sci. U.S.A. contributor: fullname: Somerville – volume: 69 start-page: 1021 year: 2005 end-page: 1024 ident: b0005 article-title: Identification of mutation sites on Δ6 desaturase genes from mortierella alpina 1S–4 mutants publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Shimizu – volume: 116 start-page: 417 year: 2013 end-page: 422 ident: b0115 article-title: Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S–4 publication-title: J. Biosci. Bioeng. contributor: fullname: Ogawa – volume: 7 start-page: 495 year: 2006 end-page: 502 ident: b0075 article-title: Mediterranean dietary traditions for the molecular treatment of human cancer: anti-oncogenic actions of the main olive oil's monounsaturated fatty acid oleic acid (18:1n–9) publication-title: Curr. Pharm. Biotechnol. contributor: fullname: Lupu – volume: 52 start-page: 3434 year: 2004 end-page: 3440 ident: b0025 article-title: Influence of harvest date and crop yield on the fatty acid composition of virgin olive oils from cv. Picual publication-title: J. Agric. Food Chem. contributor: fullname: Martínez – volume: 229 start-page: 317 year: 1995 end-page: 322 ident: b0050 article-title: Preparation of 13C-labeled polyunsaturated fatty acids by an arachidonic acid-producing fungus Mortierella alpina 1S–4 publication-title: Anal. Biochem. contributor: fullname: Shimizu – volume: 66 start-page: 648 year: 2005 end-page: 654 ident: b0100 article-title: A novel fungal ω3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S–4 publication-title: Appl. Microbiol. Biotechnol. contributor: fullname: Shimizu – volume: 60 start-page: 183 year: 2014 end-page: 191 ident: b0085 article-title: Selection and characterization of promoters based on genomic approach for the molecular breeding of oleaginous fungus Mortierella alpina 1S–4 publication-title: Curr. Genet. contributor: fullname: Ogawa – volume: 581 start-page: 315 year: 2007 end-page: 319 ident: b0185 article-title: Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, publication-title: FEBS Lett. contributor: fullname: Shimizu – volume: 1258 start-page: 251 year: 1995 end-page: 256 ident: b0060 article-title: Opposite effects on cholesterol metabolism and their mechanisms induced by dietary oleic acid and palmitic acid in hamsters publication-title: Biochim. Biophys. Acta contributor: fullname: Kajiyama – volume: 281 start-page: 36533 year: 2006 end-page: 36541 ident: b0140 article-title: A bifunctional Δ12, Δ15-desaturase from acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids publication-title: J. Biol. Chem. contributor: fullname: Napier – volume: 238 start-page: 445 year: 1999 end-page: 453 ident: b0125 article-title: Δ6-fatty acid desaturase from an arachidonic acid-producing publication-title: Gene contributor: fullname: Shimizu – volume: 22 start-page: 505 year: 2002 end-page: 531 ident: b0030 article-title: Dietary conjugated linoleic acid in health: physiological effects and mechanisms of action publication-title: Annu. Rev. Nutr. contributor: fullname: Belury – volume: 107 start-page: 99 year: 2009 end-page: 101 ident: b0105 article-title: Identification of mutation sites on Delta12 desaturase genes from Mortierella alpina 1S–4 mutants publication-title: J. Biosci. Bioeng. contributor: fullname: Shimizu – volume: 67 start-page: 1246 year: 2001 end-page: 1252 ident: b0080 article-title: Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by publication-title: Appl. Environ. Microbiol. contributor: fullname: Shimizu – volume: 116 start-page: 672 year: 2013 end-page: 676 ident: b0055 article-title: Characterization of a trifunctional fatty acid desaturase from oleaginous filamentous fungus Mortierella alpina 1S–4 using a yeast expression system publication-title: J. Biosci. Bioeng. contributor: fullname: Ogawa – volume: 74 start-page: 908 year: 2010 end-page: 917 ident: b0095 article-title: Advances in the production of various polyunsaturated fatty acids through oleaginous fungus publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Sakuradani – volume: 84 start-page: 1 year: 2009 end-page: 10 ident: b0110 article-title: Improved production of various polyunsaturated fatty acids through filamentous fungus publication-title: Appl. Microbiol. Biotechnol. contributor: fullname: Shimizu – volume: 47 start-page: 1774 year: 2008 end-page: 1778 ident: b0020 article-title: Obtaining 2-octanol, 2-octanone, and sebacic acid from castor oil by microwave-induced alkali fusion publication-title: Ind. Eng. Chem. Res. contributor: fullname: Demirel – volume: 97 start-page: 269 year: 2013 end-page: 281 ident: b0155 article-title: Increased production of fatty acids and triglycerides in publication-title: Appl. Microbiol. Biotechnol. contributor: fullname: Machida – volume: 160 start-page: 837 year: 2000 end-page: 842 ident: b0040 article-title: Olive oil and reduced need for antihypertensive medications publication-title: Arch. Intern. Med. contributor: fullname: Marotta – volume: 7 start-page: 252 year: 2002 end-page: 262 ident: b0045 article-title: Production of arachidonic acid by publication-title: Biotechnol. Bioprocess Eng. contributor: fullname: Shimizu – volume: 51 start-page: 785 year: 1987 end-page: 790 ident: b0170 article-title: Production of arachidonic acid by Mortierella elongata 1S–5 publication-title: Agric. Biol. Chem. contributor: fullname: Shinmen – volume: 70 start-page: 711 year: 2006 end-page: 719 ident: b0010 article-title: Functional characterization of Δ9 and ω9 desaturase genes in Mortierella alpina 1S–4 and its derivative mutants publication-title: Appl. Microbiol. Biotechnol. contributor: fullname: Shimizu – volume: 22 start-page: 573 year: 2013 end-page: 576 ident: b0180 article-title: Clone and identification of bifunctional Δ12/Δ15 fatty acid desaturase LKFAD15 from publication-title: Food Sci. Biotechnol. contributor: fullname: Fenghong – volume: 29 start-page: 193 year: 2015 end-page: 196 ident: b0065 article-title: Fatty acid composition and tocopherol profiles of safflower ( publication-title: Nat. Prod. Res. contributor: fullname: Al-Juhaimi – volume: 60 start-page: 281 year: 2002 end-page: 287 ident: b0120 article-title: Production of 5,8,11-eicosatrienoic acid by a Δ5 and Δ6 desaturation activity-enhanced mutant derived from a Δ12 desaturation activity-defective mutant of Mortierella alpina 1S–4 publication-title: Appl. Microbiol. Biotechnol. contributor: fullname: Shimizu – volume: 120 start-page: 299 year: 2015 end-page: 304 ident: b0090 article-title: Omega-3 eicosatetraenoic acid production by molecular breeding of the mutant strain S14 derived from Mortierella alpina 1S–4 publication-title: J. Biosci. Bioeng. contributor: fullname: Ogawa – volume: 180 start-page: 1497 year: 2016 end-page: 1507 ident: b0165 article-title: Overexpression of Δ12-fatty acid desaturase in the oleaginous yeast publication-title: Appl. Biochem. Biotechnol. contributor: fullname: Zhao – volume: 286 start-page: 43644 year: 2011 end-page: 43650 ident: b0190 article-title: Delta12-desaturase FAT-2 is a bifunctional desaturase able to desaturate a diverse range of fatty acid substrates at the Delta12 and Delta15 positions publication-title: J. Biol. Chem. contributor: fullname: Singh – volume: 75 start-page: 5529 year: 2009 ident: 10.1016/j.biortech.2017.06.089_b0015 article-title: Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S–4, and its application for eicosapentaenoic acid producer breeding publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.00648-09 contributor: fullname: Ando – volume: 74 start-page: 908 year: 2010 ident: 10.1016/j.biortech.2017.06.089_b0095 article-title: Advances in the production of various polyunsaturated fatty acids through oleaginous fungus Mortierella alpina breeding publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.100001 contributor: fullname: Sakuradani – volume: 120 start-page: 1282 year: 2016 ident: 10.1016/j.biortech.2017.06.089_b0150 article-title: Efficient enzymatic production of hydroxy fatty acids by linoleic acid Δ9 hydratase from Lactobacillus plantarum AKU 1009a publication-title: J. Appl. Microbiol. doi: 10.1111/jam.13088 contributor: fullname: Takeuchi – volume: 69 start-page: 1021 year: 2005 ident: 10.1016/j.biortech.2017.06.089_b0005 article-title: Identification of mutation sites on Δ6 desaturase genes from mortierella alpina 1S–4 mutants publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.69.1021 contributor: fullname: Abe – volume: 11 start-page: 192 year: 2014 ident: 10.1016/j.biortech.2017.06.089_b0035 article-title: Immunomodulatory activity of a gut microbial metabolite of dietary linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, associated with improved antioxidant/detoxifying defences publication-title: J. Funct. Foods doi: 10.1016/j.jff.2014.10.007 contributor: fullname: Bergamo – volume: 22 start-page: 505 year: 2002 ident: 10.1016/j.biortech.2017.06.089_b0030 article-title: Dietary conjugated linoleic acid in health: physiological effects and mechanisms of action publication-title: Annu. Rev. Nutr. doi: 10.1146/annurev.nutr.22.021302.121842 contributor: fullname: Belury – volume: 120 start-page: 299 year: 2015 ident: 10.1016/j.biortech.2017.06.089_b0090 article-title: Omega-3 eicosatetraenoic acid production by molecular breeding of the mutant strain S14 derived from Mortierella alpina 1S–4 publication-title: J. Biosci. Bioeng. doi: 10.1016/j.jbiosc.2015.01.014 contributor: fullname: Okuda – volume: 238 start-page: 445 year: 1999 ident: 10.1016/j.biortech.2017.06.089_b0125 article-title: Δ6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus: gene cloning and its heterologous expression in a fungus, Aspergillus publication-title: Gene doi: 10.1016/S0378-1119(99)00359-5 contributor: fullname: Sakuradani – volume: 281 start-page: 36533 year: 2006 ident: 10.1016/j.biortech.2017.06.089_b0140 article-title: A bifunctional Δ12, Δ15-desaturase from acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids publication-title: J. Biol. Chem. doi: 10.1074/jbc.M605158200 contributor: fullname: Sayanova – volume: 581 start-page: 315 year: 2007 ident: 10.1016/j.biortech.2017.06.089_b0185 article-title: Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-2 publication-title: FEBS Lett. doi: 10.1016/j.febslet.2006.12.031 contributor: fullname: Zhang – volume: 60 start-page: 183 year: 2014 ident: 10.1016/j.biortech.2017.06.089_b0085 article-title: Selection and characterization of promoters based on genomic approach for the molecular breeding of oleaginous fungus Mortierella alpina 1S–4 publication-title: Curr. Genet. doi: 10.1007/s00294-014-0423-4 contributor: fullname: Okuda – volume: 70 start-page: 711 year: 2006 ident: 10.1016/j.biortech.2017.06.089_b0010 article-title: Functional characterization of Δ9 and ω9 desaturase genes in Mortierella alpina 1S–4 and its derivative mutants publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-005-0115-6 contributor: fullname: Abe – volume: 71 start-page: 5124 year: 2005 ident: 10.1016/j.biortech.2017.06.089_b0145 article-title: Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S–4, through RNA interference with Δ12-desaturase gene expression publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.71.9.5124-5128.2005 contributor: fullname: Takeno – volume: 15 start-page: 314 year: 2015 ident: 10.1016/j.biortech.2017.06.089_b0175 article-title: Review of the roles of conjugated linoleic acid in health and disease publication-title: J. Funct. Foods doi: 10.1016/j.jff.2015.03.050 contributor: fullname: Yang – volume: 7 start-page: 495 year: 2006 ident: 10.1016/j.biortech.2017.06.089_b0075 article-title: Mediterranean dietary traditions for the molecular treatment of human cancer: anti-oncogenic actions of the main olive oil's monounsaturated fatty acid oleic acid (18:1n–9) publication-title: Curr. Pharm. Biotechnol. doi: 10.2174/138920106779116900 contributor: fullname: Menendez – volume: 286 start-page: 43644 year: 2011 ident: 10.1016/j.biortech.2017.06.089_b0190 article-title: Caenorhabditis elegans Delta12-desaturase FAT-2 is a bifunctional desaturase able to desaturate a diverse range of fatty acid substrates at the Delta12 and Delta15 positions publication-title: J. Biol. Chem. doi: 10.1074/jbc.M111.266114 contributor: fullname: Zhou – volume: 160 start-page: 837 year: 2000 ident: 10.1016/j.biortech.2017.06.089_b0040 article-title: Olive oil and reduced need for antihypertensive medications publication-title: Arch. Intern. Med. doi: 10.1001/archinte.160.6.837 contributor: fullname: Ferrara – volume: 47 start-page: 1774 year: 2008 ident: 10.1016/j.biortech.2017.06.089_b0020 article-title: Obtaining 2-octanol, 2-octanone, and sebacic acid from castor oil by microwave-induced alkali fusion publication-title: Ind. Eng. Chem. Res. doi: 10.1021/ie071345u contributor: fullname: Azcan – volume: 51 start-page: 785 year: 1987 ident: 10.1016/j.biortech.2017.06.089_b0170 article-title: Production of arachidonic acid by Mortierella elongata 1S–5 publication-title: Agric. Biol. Chem. contributor: fullname: Yamada – volume: 52 start-page: 3434 year: 2004 ident: 10.1016/j.biortech.2017.06.089_b0025 article-title: Influence of harvest date and crop yield on the fatty acid composition of virgin olive oils from cv. Picual publication-title: J. Agric. Food Chem. doi: 10.1021/jf049894n contributor: fullname: Beltrán – volume: 7 start-page: 252 year: 2002 ident: 10.1016/j.biortech.2017.06.089_b0045 article-title: Production of arachidonic acid by Mortierella fungi publication-title: Biotechnol. Bioprocess Eng. doi: 10.1007/BF02932833 contributor: fullname: Higashiyama – volume: 107 start-page: 99 year: 2009 ident: 10.1016/j.biortech.2017.06.089_b0105 article-title: Identification of mutation sites on Delta12 desaturase genes from Mortierella alpina 1S–4 mutants publication-title: J. Biosci. Bioeng. doi: 10.1016/j.jbiosc.2008.10.011 contributor: fullname: Sakuradani – volume: 84 start-page: 1 year: 2009 ident: 10.1016/j.biortech.2017.06.089_b0110 article-title: Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-009-2076-7 contributor: fullname: Sakuradani – volume: 18 start-page: 6743 year: 1995 ident: 10.1016/j.biortech.2017.06.089_b0160 article-title: An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.92.15.6743 contributor: fullname: van de Loo – volume: 66 start-page: 648 year: 2005 ident: 10.1016/j.biortech.2017.06.089_b0100 article-title: A novel fungal ω3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S–4 publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-004-1760-x contributor: fullname: Sakuradani – volume: 147 start-page: 1325 year: 2008 ident: 10.1016/j.biortech.2017.06.089_b0070 article-title: An oleate hydroxylase from the fungus Claviceps purpurea: cloning, functional analysis, and expression in Arabidopsis publication-title: Plant Physiol. doi: 10.1104/pp.108.117168 contributor: fullname: Meesapyodsuk – volume: 229 start-page: 317 year: 1995 ident: 10.1016/j.biortech.2017.06.089_b0050 article-title: Preparation of 13C-labeled polyunsaturated fatty acids by an arachidonic acid-producing fungus Mortierella alpina 1S–4 publication-title: Anal. Biochem. doi: 10.1006/abio.1995.1419 contributor: fullname: Kawashima – volume: 1258 start-page: 251 year: 1995 ident: 10.1016/j.biortech.2017.06.089_b0060 article-title: Opposite effects on cholesterol metabolism and their mechanisms induced by dietary oleic acid and palmitic acid in hamsters publication-title: Biochim. Biophys. Acta doi: 10.1016/0005-2760(95)00122-S contributor: fullname: Kurushima – volume: 60 start-page: 281 year: 2002 ident: 10.1016/j.biortech.2017.06.089_b0120 article-title: Production of 5,8,11-eicosatrienoic acid by a Δ5 and Δ6 desaturation activity-enhanced mutant derived from a Δ12 desaturation activity-defective mutant of Mortierella alpina 1S–4 publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-002-1128-z contributor: fullname: Sakuradani – volume: 116 start-page: 672 year: 2013 ident: 10.1016/j.biortech.2017.06.089_b0055 article-title: Characterization of a trifunctional fatty acid desaturase from oleaginous filamentous fungus Mortierella alpina 1S–4 using a yeast expression system publication-title: J. Biosci. Bioeng. doi: 10.1016/j.jbiosc.2013.05.023 contributor: fullname: Kikukawa – volume: 29 start-page: 193 year: 2015 ident: 10.1016/j.biortech.2017.06.089_b0065 article-title: Fatty acid composition and tocopherol profiles of safflower (Carthamus tinctorius L.) seed oils publication-title: Nat. Prod. Res. doi: 10.1080/14786419.2014.971316 contributor: fullname: Matthaus – volume: 67 start-page: 704 year: 2003 ident: 10.1016/j.biortech.2017.06.089_b0135 article-title: Gene cloning and functional analysis of a second Δ6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.67.704 contributor: fullname: Sakuradani – volume: 22 start-page: 573 year: 2013 ident: 10.1016/j.biortech.2017.06.089_b0180 article-title: Clone and identification of bifunctional Δ12/Δ15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae publication-title: Food Sci. Biotechnol. doi: 10.1007/s10068-013-0116-7 contributor: fullname: Yan – volume: 67 start-page: 1246 year: 2001 ident: 10.1016/j.biortech.2017.06.089_b0080 article-title: Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.67.3.1246-1252.2001 contributor: fullname: Ogawa – volume: 97 start-page: 269 year: 2013 ident: 10.1016/j.biortech.2017.06.089_b0155 article-title: Increased production of fatty acids and triglycerides in Aspergillus oryzae by enhancing expressions of fatty acid synthesis-related genes publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-012-4193-y contributor: fullname: Tamano – volume: 180 start-page: 1497 year: 2016 ident: 10.1016/j.biortech.2017.06.089_b0165 article-title: Overexpression of Δ12-fatty acid desaturase in the oleaginous yeast Rhodosporidium toruloides for production of linoleic acid-rich lipids publication-title: Appl. Biochem. Biotechnol. doi: 10.1007/s12010-016-2182-9 contributor: fullname: Wang – volume: 81 start-page: 497 year: 2008 ident: 10.1016/j.biortech.2017.06.089_b0130 article-title: Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S–4 publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-008-1675-z contributor: fullname: Sakuradani – volume: 116 start-page: 417 year: 2013 ident: 10.1016/j.biortech.2017.06.089_b0115 article-title: Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S–4 publication-title: J. Biosci. Bioeng. doi: 10.1016/j.jbiosc.2013.04.008 contributor: fullname: Sakuradani |
| SSID | ssj0003172 |
| Score | 2.4118302 |
| Snippet | •LA or OA high-producing strains were obtained by molecular breeding of M. alpina.•The production of LA and OA reached 3.32 and 3.85g/L, respectively, on the... The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA).... |
| SourceID | osti proquest crossref pubmed elsevier |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 1610 |
| SubjectTerms | DNA Shuffling Fatty acid desaturase Fatty Acid Desaturases Linoleic acid Linoleic Acids Metabolic Engineering Mortierella Mortierella alpina Oleic acid RNAi |
| Title | Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids |
| URI | https://dx.doi.org/10.1016/j.biortech.2017.06.089 https://www.ncbi.nlm.nih.gov/pubmed/28673516 https://search.proquest.com/docview/1915878591 https://www.osti.gov/biblio/2396013 |
| Volume | 245 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9QwDLe22wPwgGB83QZTkHjt7to0SfN4OjEdoO0FJu0tStJk6nRrT9vtlb99dpuOIYF44KlKUleR7dq_yI4N8Ek4hyhZ6kzpGLKyKOtMO6-yWGivylJHyemi8OmZXJ2XXy_ExQ4sx7swlFaZbP9g03trnWZmiZuzTdPMvhP4rgR6IFRS9LLVLuz1QaIJ7C2-fFudPRhkdJF9MAHfz4jg0UXhq2PXUFJrH5fIVV_Kkzq-_9lHTTr87f4ORXuXdPICnicsyRbDdl_CTmj34dni8ibV0wj78GQ5NnTDlUe1B1_B9WnYogKsG8_Cr3nWRdatA7Uu6u5uGXq9S3xQRi5ujRKlmF1vmtYyxLqMSh2zzVAyFsWbaPGDtq0Zwtc08E19-xrOTz7_WK6y1Hsh80LPt5l1vKqdzKsgfa2dtkEV3Ape6xiDQibiOcTb4BAvRMHl3PMYCY4Ucx0LETh_A5O2a8M7YHmUWsRQCY9kvtQuRlsqqSyClaBDPYXZyG2zGUpsmDH37MqM8jEkH0NJeJWegh6FYn5TFoN-4J-0hyRFoqMlT-lESFhw1I6cT-HjKFyD0qHgiW0DMtzguVZUiqr9TeHtIPWH3RaVVFzk8uA_9nUIT2k0ZMq8h8n25i58QLyzdUewe_wzP0pafQ_q_gDX |
| link.rule.ids | 230,315,783,787,888,4511,24130,27938,27939,45599,45693 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT9wwEB7R5UB7qFr62tKHkbiG3cSxHR_Rqmh5LJeCxM2yHRsFLckKlmt_e2fyKCBR9dBTFNsTWf4mns_y5zHAnnAOWbLUidIxJHmWl4l2XiUx017luY6S00HhxZmcX-THl-JyA2bDWRiSVfZzfzent7N1XzLpR3OyqqrJTyLfhcAIhE6KUbZ4AZs55c9Cp97_9aDzwADZbiVg64SaPzomfL3vKpK0trsSqWoTedJ9789HqFGDP93fiWgbkA7fwOueSbKDrrNvYSPU2_Dq4Oq2z6YRtmFrNlznhjWPMg--g5tFWCP8y8qz8FDOmsiaZaCLi5r7O4Yx7wofpMfFrpFMitnlqqotQ6bLKNExW3UJYxHc3hY_aOuSIXntX3xV3r2Hi8Mf57N50t-8kHihp-vEOl6UTqZFkL7UTtugMm4FL3WMQeEg4irE2-CQLUTB5dTzGImMZFMdMxE4_wCjuqnDJ2BplFrEUAiPZj7XLkabK6ksUpWgQzmGyTDaZtUl2DCD8uzaDPgYwseQBK_QY9ADKOaJqxiMAv-03SEUyY6qPImJ0DDj6B0pH8PuAK5BdGjrxNYBB9zgqlYUinL9jeFjh_qf3maFVFyk8vN_9Os7bM3PF6fm9OjsZAdeUk2nmfkCo_XtffiKzGftvrWe_RtduQG5 |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Metabolic+engineering+of+oleaginous+fungus+Mortierella+alpina+for+high+production+of+oleic+and+linoleic+acids&rft.jtitle=Bioresource+technology&rft.au=Sakamoto%2C+Takaiku&rft.au=Sakuradani%2C+Eiji&rft.au=Okuda%2C+Tomoyo&rft.au=Kikukawa%2C+Hiroshi&rft.date=2017-12-01&rft.eissn=1873-2976&rft.volume=245&rft.spage=1610&rft.epage=1615&rft_id=info:doi/10.1016%2Fj.biortech.2017.06.089&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0960-8524&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0960-8524&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0960-8524&client=summon |