Genome-Wide Single Nucleotide Polymorphism Typing Method for Identification of Bacillus anthracis Species and Strains among B. cereus Group Species

• Published in: Journal of Clinical Microbiology Vol. 48; no. 8; pp. 2821 - 2829
• Main Authors: Kuroda, Makoto, Serizawa, Masakuni, Okutani, Akiko, Sekizuka, Tsuyoshi, Banno, Satomi, Inoue, Satoshi
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2010; American Society for Microbiology (ASM)
• Subjects: Bacillus anthracis; Bacillus anthracis - classification; Bacillus anthracis - genetics; Bacillus anthracis - isolation & purification; Bacillus cereus; Bacillus cereus - classification; Bacillus cereus - genetics; Bacillus cereus - isolation & purification; Bacterial Typing Techniques; Bacterial Typing Techniques - methods; Bacteriology; Biological and medical sciences; Chromosomes, Bacterial; DNA, Bacterial; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Genome, Bacterial; Genotype; Humans; Japan; Microbiology; Miscellaneous; Phylogeny; Plasmids; Polymerase Chain Reaction; Polymerase Chain Reaction - methods; Polymorphism, Single Nucleotide; Japan; Bacillus anthracis; Typing; Bacillales; Bacteria; Identification; Method; Single nucleotide polymorphism; Bacillaceae; Genome; Strain

As an issue of biosecurity, species-specific genetic markers have been well characterized. However, Bacillus anthracis strain-specific information is currently not sufficient for traceability to identify the origin of the strain. By using genome-wide screening using short read mapping, we identified...