A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans

• Published in: PLoS genetics Vol. 21; no. 1; p. e1011541
• Main Authors: Khan, Haania, Huang, Xinyu, Raj, Vishnu, Wang, Han
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.01.2025; Public Library of Science (PLoS)
• Subjects: Animals; Animals, Genetically Modified; Biology and Life Sciences; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans Proteins - genetics; CRISPR-Cas Systems - genetics; Gene Editing - methods; Gene expression; Gene Expression Regulation; Genetic aspects; Genetic engineering; Genetic research; Methods; Operon; Research and Analysis Methods; Transgenes; United States

The ability to manipulate gene activity and control transgene expression is essential to study gene function. While several genetic tools for modifying genes or controlling expression separately are available for Caenorhabditis elegans , there are no genetic approaches to generate mutations that sim...