Effects of Adenosine Dialdehyde Treatment on In Vitro and In Vivo Stable Protein Methylation in HeLa Cells

• Published in: Journal of biochemistry (Tokyo) Vol. 136; no. 3; pp. 371 - 376
• Main Authors: Chen, Da-Huang, Wu, Kuan-Tsu, Hung, Chien-Jen, Hsieh, Mingli, Li, Chuan
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.09.2004
• Subjects: Adenosine - analogs & derivatives; Adenosine - chemistry; Adenosine - pharmacology; adenosine dialdehyde; aDMA; AdoHcy; AdoMet; AdOx; ALLN; Arginine - chemistry; asymmetric dimethylarginin; Blotting, Western; CHX; cycloheximide; Cycloheximide - chemistry; Cycloheximide - pharmacology; Detergents - pharmacology; DNA Methylation; HeLa Cells; Humans; in vivo methylation; Methylation; MMA; monomethylarginine; N-acetyl-leu-leu-norleucinal; Oxygen - metabolism; Peptides - chemistry; PRMT; Proteasome Endopeptidase Complex - chemistry; protein arginine methyltransferase; protein methylation; Protein Synthesis Inhibitors - pharmacology; Proteins - chemistry; S-adenosyl-l-homocystein; S-adenosylmethionine; sDMA; symmetric dimethylarginine; Time Factors; Trypsin - pharmacology

Adenosine dialdehyde (AdOx) is an indirect methyltransferase inhibitor broadly used in cell culture to accumulate methyl-accepting proteins in hypomethylated states for in vitro protein methylation analyses. In this study we included a translation inhibitor, cycloheximide, in the AdOx treatment of H...