Redox proteomic identification of HNE-bound mitochondrial proteins in cardiac tissues reveals a systemic effect on energy metabolism after doxorubicin treatment

• Published in: Free radical biology & medicine Vol. 72; pp. 55 - 65
• Main Authors: Zhao, Y., Miriyala, S., Miao, L., Mitov, M., Schnell, D., Dhar, S.K., Cai, J., Klein, J.B., Sultana, R., Butterfield, D.A., Vore, M., Batinic-Haberle, I., Bondada, S., St. Clair, D.K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2014
• Subjects: Aldehydes - metabolism; Animals; Antibiotics, Antineoplastic - toxicity; ATP synthase; Cardiac injury; Dihydrolipoyl dehydrogenase; Doxorubicin; Doxorubicin - toxicity; Electrophoresis, Gel, Two-Dimensional; Energy Metabolism - drug effects; Free radicals; Immunoblotting; Immunoprecipitation; Lipid Peroxidation - drug effects; Male; Metabolism; Mice; Mice, Inbred C57BL; Mitochondria, Heart - drug effects; Mitochondria, Heart - metabolism; NADH dehydrogenase [ubiquinone] iron–sulfur protein 2; Oxidation-Reduction; Oxidative stress; Proteomics; Redox proteomics; Succinate dehydrogenase [ubiquinone] flavoprotein; Oxidative stress; FH; Doxorubicin; CKMT2; ATP5B; ATP synthase; Succinate dehydrogenase [ubiquinone] flavoprotein; Free radicals; Cardiac injury; TCA cycle; Oxct1; Dihydrolipoyl dehydrogenase; DOX; SDHA; Metabolism; MnP; ECAR; HADHA; NAC; Redox proteomics; NADH dehydrogenase [ubiquinone] iron–sulfur protein 2; ETC; ROS; NDUFS2; DLD; HNE; OCR

Doxorubicin (DOX), one of the most effective anticancer drugs, is known to generate progressive cardiac damage, which is due, in part, to DOX-induced reactive oxygen species (ROS). The elevated ROS often induce oxidative protein modifications that result in alteration of protein functions. This stud...