桂野生山金柑的倍性分析和SSR分子鉴定
【目的】研究‘桂野生山金柑’的遗传学特性,为柑橘种质资源的鉴定及创新利用提供理论依据。【方法】采用倍性分析和SSR标记相结合,对‘桂野生山金柑’进行遗传鉴定分析。【结果】倍性分析结果表明‘桂野生山金柑’是二倍体;从24对SSR引物中筛选出6对重复性好、条带清晰、多态性强的核基因组引物,在供试材料中获得等位基因条带共30条,其中具有多样性差异条带25条,多态性比例为79.17%。聚类分析的结果表明不同供试材料的遗传相似系数在0.600-0.802。【结论】‘桂野生山金柑’具有遗传特异性,不同于其他地区起源的山金柑或金柑,是一种新的柑橘种质资源。...
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| Published in | 西南农业学报 Vol. 30; no. 8; pp. 1872 - 1876 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西农业科学院园艺研究所/农业部南宁南亚热带果树科学观测实验站,广西南宁,530007%华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,湖北武汉,430070%东兴市农业技术推广中心,广西东兴,538100
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.16213/j.cnki.scjas.2017.8.030 |
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| Abstract | 【目的】研究‘桂野生山金柑’的遗传学特性,为柑橘种质资源的鉴定及创新利用提供理论依据。【方法】采用倍性分析和SSR标记相结合,对‘桂野生山金柑’进行遗传鉴定分析。【结果】倍性分析结果表明‘桂野生山金柑’是二倍体;从24对SSR引物中筛选出6对重复性好、条带清晰、多态性强的核基因组引物,在供试材料中获得等位基因条带共30条,其中具有多样性差异条带25条,多态性比例为79.17%。聚类分析的结果表明不同供试材料的遗传相似系数在0.600-0.802。【结论】‘桂野生山金柑’具有遗传特异性,不同于其他地区起源的山金柑或金柑,是一种新的柑橘种质资源。 |
|---|---|
| AbstractList | S666; [目的]研究‘桂野生山金柑’的遗传学特性,为柑橘种质资源的鉴定及创新利用提供理论依据.[方法]采用倍性分析和SSR标记相结合,对‘桂野生山金柑’进行遗传鉴定分析.[结果]倍性分析结果表明‘桂野生山金柑’是二倍体;从24对SSR引物中筛选出6对重复性好、条带清晰、多态性强的核基因组引物,在供试材料中获得等位基因条带共30条,其中具有多样性差异条带25条,多态性比例为79.17％.聚类分析的结果表明不同供试材料的遗传相似系数在0.600-0.802.[结论]‘桂野生山金柑’具有遗传特异性,不同于其他地区起源的山金柑或金柑,是一种新的柑橘种质资源. 【目的】研究‘桂野生山金柑’的遗传学特性,为柑橘种质资源的鉴定及创新利用提供理论依据。【方法】采用倍性分析和SSR标记相结合,对‘桂野生山金柑’进行遗传鉴定分析。【结果】倍性分析结果表明‘桂野生山金柑’是二倍体;从24对SSR引物中筛选出6对重复性好、条带清晰、多态性强的核基因组引物,在供试材料中获得等位基因条带共30条,其中具有多样性差异条带25条,多态性比例为79.17%。聚类分析的结果表明不同供试材料的遗传相似系数在0.600-0.802。【结论】‘桂野生山金柑’具有遗传特异性,不同于其他地区起源的山金柑或金柑,是一种新的柑橘种质资源。 |
| Abstract_FL | [Objective] In order to provide a theoretical basis for the identification and utilization of Gui Wild Shanjingan,its genetic characteristics was studied [Methods] Using ploidy analysis and SSR molecular marker to analyze the genetic diversity of Gui Wild Shanjingan.[Results] The ploidy analysis result showed that Gui Wild Shanjingan was diploid.6 pairs of SSR primers with good repeatability,high polymorphism and clear bands were screened from the 24 pairs of SSR primers.A total of 30 allele stripes were successfully observed,among them,25 bands were polymorphism.The polymorphic rate was 79.17 ％.Genetic similarities caculated by cluster analysis ranged from 0.600-0.802.[Conclusion] Gui Wild Shanjingan was significant different from Shanjingan or Jingan originated from other origions,it was a new cultivar with genetic specificity. |
| Author | 李果果 刘要鑫 柴利军 叶俊丽 麦彩胜 欧智涛 陈香玲 |
| AuthorAffiliation | 广西农业科学院园艺研究所／农业部南宁南亚热带果树科学观测实验站,广西南宁530007 华中农业大学园艺林学学院／园艺植物生物学教育部重点实验室,湖北武汉430070 东兴市农业技术推广中心,广西东兴538100 |
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| Author_FL | YE Jun-li LI Guo-guo LIU Yao-xin CHAI Li-jun CHEN Xiang-ling OU Zhi-tao MAI Cai-sheng |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.16213/j.cnki.scjas.2017.8.030 |
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| DocumentTitleAlternate | Ploidy Analysis and SSR Molecular Identification of Gui Wild Shanjingan |
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| Keywords | Gui Wild Shanjingan Cluster analysis SSR molecular marker 倍性分析 Ploidy analysis 桂野生山金柑 SSR分子标记 聚类分析 |
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| Notes | Gut Wild Shanjingan ; Ploidy analysis ; SSR molecular marker ; Cluster analysis 51-1213/S LI Guo-guo1 , LIU Yao-xin1 , CHAI Li-jun2 , YE Jun-li2 , MAI Cai-sheng3 , OU Zhi-tao1 ,CHEN Xiang-ling1 （ 1. Horticulture Research Institute, Guangxi Academy of Agriculture Sciences/Nanning Investigation ＆ Experiment Station of South Subtropical Fruit Trees, Ministry of Agriculture, Guangxi Nanning 530007, China; 2. College of Horticulture ＆ Forestry Sciences, Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology, Ministry of Education, Hubei Wuhan 430070, China; 3. Agricuhural Technology Extension Center of Dongxing City, Guangxi Dongxing 538100,China） Objective] In order to provide a theoretical basis for the identification and utilization of Gut Wild Shanjingan, its genetic charac-teristics was studied [ Methodsl Using ploidy analysis and SSR molecular marker to analyze the genetic diversity of Gut Wild Shanjingan. [ Results] The ploidy analysis result showed that Gut Wild shanjingan was diploid. |
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| SubjectTerms | SSR分子标记 倍性分析 桂野生山金柑 聚类分析 |
| Title | 桂野生山金柑的倍性分析和SSR分子鉴定 |
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