Ozone primes alveolar macrophage–derived innate immunity in healthy human subjects

• Published in: Journal of allergy and clinical immunology Vol. 138; no. 4; pp. 1213 - 1215.e1
• Main Authors: Frush, Benjamin W., Li, Zhuowei, Stiles, Jane V., Cotter, Sarah F., Shofer, Scott L., Foster, W. Michael, Hollingsworth, John W., Tighe, Robert M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2016; Elsevier Limited
• Subjects: Administration, Inhalation; Adult; Allergy and Immunology; Animals; Body mass index; Female; Genes; Healthy Volunteers; Human subjects; Humans; Immunity, Innate - drug effects; Immunity, Innate - genetics; Immunity, Innate - physiology; In Vitro Techniques; Kinases; Laboratories; Lipopolysaccharide Receptors - genetics; Lipopolysaccharides - pharmacology; Macrophages, Alveolar - drug effects; Macrophages, Alveolar - immunology; Macrophages, Alveolar - metabolism; Male; Mice; Ozone - administration & dosage; Ozone - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Tetradecanoylphorbol Acetate - pharmacology; Toll-Like Receptor 4 - genetics; Young Adult

Consistent with animal studies, evidence from sputum macrophages isolated from humans exposed to ozone identified enhanced surface expression of TLR4 and CD14 by flow cytometry.2-4 However, the functional relevance of these initial observations in healthy human subjects remained unknown. [...]the pr...