Blood-brain barrier leakage of blood proteins in idiopathic normal pressure hydrocephalus

• Published in: Brain research Vol. 1727; p. 146547
• Main Authors: Eide, Per Kristian, Hansson, Hans-Arne
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2020
• Subjects: Adult; Aged; aquaporin-4; association; Astrogliosis; Blood Proteins - analysis; Blood-Brain Barrier - pathology; Blood-brain barrier dysfunction; Cerebral Cortex - pathology; disease; Dysfunction of capillaries; Extravasation of Diagnostic and Therapeutic Materials; Female; Fibrin - analysis; fibrinogen; Fibrinogen/fibrin extravasation; fibrinolysis; flow; health; Humans; Hydrocephalus, Normal Pressure - pathology; Immunohistochemistry; Impaired neurovascular unit; Male; management; Microscopy; Middle Aged; Neurodegeneration; Neurologi; Neurology; Neurosciences; Neurosciences & Neurology; neurovascular unit; Neurovetenskaper; permeability; Astrogliosis; Blood-brain barrier dysfunction; Impaired neurovascular unit; Fibrinogen/fibrin extravasation; Dysfunction of capillaries; Neurodegeneration
• DOI: 10.1016/j.brainres.2019.146547
• ISSN: 0006-8993; 1872-6240

•BBB leakage of blood proteins was examined in iNPH and controls.•Light microscopy revealed fibrin(ogen) extravasation in iNPH.•Degree of fibrin(ogen) extravasation and astrogliosis was positively correlated.•Degree of fibrin(ogen) extravasation and aquaporin-4 and dystrophin-71 was correlated.•BBB dysfunction may be part of pathophysiology of iNPH. Idiopathic normal pressure hydrocephalus (iNPH) is one subtype of dementia characterized by cerebrospinal fluid (CSF) disturbance, but with unknown cause. We recently reported that frontal cortex biopsies of iNPH patients disclosed degenerative alterations of the capillary basement membrane, including degenerated pericyte processes. Given that pericyte degeneration is associated with blood-brain barrier (BBB) dysfunction, the present study was undertaken to examine whether BBB leakage of blood proteins can be revealed by light microscopy (LM) immunohistochemistry in iNPH. The study included cortical brain tissue specimens from 14 reference (REF) subjects undergoing neurosurgery for epilepsy, aneurysm or tumor, and 45 iNPH patients. Dysfunction of the BBB was measured semi-quantitatively as area percentage extravasated fibrin(ogen) in cerebral cortical layers I, II and III. The degree of fibrin(ogen) extravasation was also correlated with expression of glial fibrillary acidic protein (GFAP), aquaporin-4 (AQP4), dystrophin 71 (Dp71) and Cluster of Differentiation 68 (CD68). The study disclosed extravasation of fibrin(ogen) in 4/14 REF subjects and in 45/45 iNPH patients, the percentage area of fibrin(ogen) was significantly higher in iNPH than REF cortical specimens. Diffuse, less prominent fibrin(ogen) extravasation was seen in the subcortical white matter of one iNPH individual. Increasing degree of fibrinogen extravasation in cerebral cortex was significantly associated with increasing degree of astrogliosis and with reduced expression of perivascular AQP4 and Dp71. The present results provide evidence of BBB dysfunction in iNPH. The BBB leakage of blood proteins may render for impaired neurovascular units in iNPH patients.