Regulation of mRNA Expression of MDR1, MRP1, MRP2 and MRP3 by Prototypical Microsomal Enzyme Inducers in Primary Cultures of Human and Rat Hepatocytes

• Published in: DRUG METABOLISM AND PHARMACOKINETICS Vol. 21; no. 4; pp. 297 - 307
• Main Authors: Nishimura, Masuhiro, Koeda, Akiko, Suzuki, Emako, Kawano, Yuichi, Nakayama, Mitsuo, Satoh, Tetsuo, Narimatsu, Shizuo, Naito, Shinsaku
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2006; Japanese Society for the Study of Xenobiotics
• Subjects: Animals; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics; Cells, Cultured; Dexamethasone - pharmacology; Enzyme Induction - drug effects; Enzyme Inhibitors; Female; Gene Expression Regulation - drug effects; hepatocytes; Hepatocytes - drug effects; Hepatocytes - metabolism; human; Humans; induction; Membrane Transport Proteins - genetics; Microsomes - drug effects; Microsomes - enzymology; Multidrug Resistance-Associated Proteins - genetics; Omeprazole - pharmacology; rat; Rats; Rats, Sprague-Dawley; Rifampin - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription, Genetic - drug effects; Transcription, Genetic - genetics; transporter; induction; rat; human; hepatocytes; transporter

The mRNA induction of various transporters by rifampicin (Rif), dexamethasone (Dex) and omeprazole (Ome) was investigated in primary cultures of cryopreserved human and rat hepatocytes. Analysis was performed by quantitative real­time RT­PCR using primers and TaqMan probes. In primary cultures of hu...