Comparison of Inducibility of CYP1A and CYP3A mRNAs by Prototypical Inducers in Primary Cultures of Human, Cynomolgus Monkey, and Rat Hepatocytes

• Published in: DRUG METABOLISM AND PHARMACOKINETICS Vol. 22; no. 3; pp. 178 - 186
• Main Authors: Nishimura, Masuhiro, Koeda, Akiko, Suganuma, Yasuyuki, Suzuki, Emako, Shimizu, Takefumi, Nakayama, Mitsuo, Satoh, Tetsuo, Narimatsu, Shizuo, Naito, Shinsaku
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2007; Japanese Society for the Study of Xenobiotics
• Subjects: Adult; Aged; Animals; Aryl Hydrocarbon Hydroxylases - genetics; Cells, Cultured; Child, Preschool; cynomolgus monkey; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP1A2 - genetics; Cytochrome P-450 CYP3A - genetics; Cytochrome P-450 Enzyme System - genetics; cytochrome P450; Dexamethasone - pharmacology; Female; Gene Expression Regulation, Enzymologic; hepatocytes; Hepatocytes - cytology; Hepatocytes - drug effects; Hepatocytes - metabolism; human; Humans; induction; Macaca fascicularis; Male; Omeprazole - pharmacology; rat; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Rifampin - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; induction; cynomolgus monkey; cytochrome P450; rat; human; hepatocytes

This study was conducted to investigate the effects of treatment with the prototypical inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on the mRNA levels of drug-metabolizing enzymes in primary cultures of cryopreserved human, cynomolgus monkey, and rat hepatocytes. Analysis was...