Molecular Characterization of Potential Nitrogen Fixation by Anaerobic Methane-Oxidizing Archaea in the Methane Seep Sediments at the Number 8 Kumano Knoll in the Kumano Basin, Offshore of Japan

• Published in: Applied and Environmental Microbiology Vol. 75; no. 22; pp. 7153 - 7162
• Main Authors: Miyazaki, Junichi, Higa, Ryosaku, Toki, Tomohiro, Ashi, Juichiro, Tsunogai, Urumu, Nunoura, Takuro, Imachi, Hiroyuki, Takai, Ken
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.11.2009; American Society for Microbiology (ASM)
• Subjects: Anaerobiosis; Archaea; Archaea - classification; Archaea - enzymology; Archaea - genetics; Archaea - metabolism; Basins; Biological and medical sciences; Cloning, Molecular; DNA, Archaeal - genetics; Fundamental and applied biological sciences. Psychology; Genes, Archaeal - genetics; Genetic diversity; Genetic Variation; Genetics; Geologic Sediments - microbiology; Japan; Methane; Methane - metabolism; Microbial Ecology; Microbiology; Molecular Sequence Data; Multigene Family - genetics; Nitrogen; Nitrogen Fixation - genetics; Oxidation; Oxidoreductases - genetics; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - chemistry; Sediments; Sequence Alignment; Asia; Japan; Characterization; Methane; Anaerobe; Archaeobacteria; Nitrogen fixation; Bacteria; Oxidation; Sediments

The potential for microbial nitrogen fixation in the anoxic methane seep sediments in a mud volcano, the number 8 Kumano Knoll, was characterized by molecular phylogenetic analyses. A total of 111 of the nifH (a gene coding a nitrogen fixation enzyme, Fe protein) clones were obtained from different...