Early identification of antigen-specific immune responses in vivo by [18F]-labeled 3'-fluoro-3'-deoxy-thymidine ([18F]FLT) PET imaging

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 45; pp. 18396 - 18399
• Main Authors: Aarntzen, Erik H. J. G, Srinivas, Mangala, De Wilt, Johannes H. W, Jacobs, Joannes F. M, Lesterhuis, W. Joost, Windhorst, Albert D, Troost, Esther G, Bonenkamp, Johannes J, van Rossum, Michelle M, Blokx, Willeke A. M, Mus, Roel D, Boerman, Otto C, Punt, Cornelis J. A, Figdor, Carl G, Oyen, Wim J. G, de Vries, I. Jolanda M
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 08.11.2011; National Acad Sciences
• Subjects: Antibodies; Antigens; Antigens, Neoplasm - immunology; Biological Sciences; biomarkers; Cancer; Cancer Vaccines - administration & dosage; Cancer Vaccines - immunology; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD8 antigen; CD8-positive T-lymphocytes; CD8-Positive T-Lymphocytes - immunology; Cell activation; Cell proliferation; Computed tomography; decision making; Dendritic cells; Dideoxynucleosides; Fluorodeoxyglucose F18; Humans; image analysis; Imaging; Immune response; Immune system; Immunization; Immunoglobulin G; immunohistochemistry; Infectious diseases; Kinetics; lymph; Lymph nodes; Lymphatic Metastasis - diagnostic imaging; Lymphatic Metastasis - immunology; Lymphocytes; Lymphocytes T; Medical treatment; Melanoma; Melanoma - diagnostic imaging; Melanoma - immunology; Melanoma - pathology; Metastases; metastasis; patients; Peripheral blood; physicians; Positron emission tomography; Positron-Emission Tomography - methods; Radiopharmaceuticals; T cell receptors; T lymphocytes; Tomography; Tracers; Vaccination; vaccine development; Vaccines

Current biomarkers are unable to adequately predict vaccine-induced immune protection in humans with infectious disease or cancer. However, timely and adequate assessment of antigen-specific immune responses is critical for successful vaccine development. Therefore, we have developed a method for th...