An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming

• Published in: Cell Vol. 147; no. 1; pp. 132 - 146
• Main Authors: Gabut, Mathieu, Samavarchi-Tehrani, Payman, Wang, Xinchen, Slobodeniuc, Valentina, O'Hanlon, Dave, Sung, Hoon-Ki, Alvarez, Manuel, Talukder, Shaheynoor, Pan, Qun, Mazzoni, Esteban O., Nedelec, Stephane, Wichterle, Hynek, Woltjen, Knut, Hughes, Timothy R., Zandstra, Peter W., Nagy, Andras, Wrana, Jeffrey L., Blencowe, Benjamin J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.09.2011; Elsevier
• Subjects: Alternative Splicing; Animals; Cellular Reprogramming; Differentiation; DNA - metabolism; Embryo cells; embryonic stem cells; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Evolution; Forkhead protein; Forkhead Transcription Factors - metabolism; Foxp1 protein; Gene expression; Gene Expression Regulation, Developmental; genes; Genes, Homeobox; Humans; induced pluripotent stem cells; Life Sciences; Mice; Oct-4 protein; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; Protein Isoforms - metabolism; proteomics; Repressor Proteins - metabolism; Somatic cells; Stem cells; transcription (genetics); Transcription factors

Alternative splicing (AS) is a key process underlying the expansion of proteomic diversity and the regulation of gene expression. Here, we identify an evolutionarily conserved embryonic stem cell (ESC)-specific AS event that changes the DNA-binding preference of the forkhead family transcription fac...