Multicenter assessment of microbial community profiling using 16S rRNA gene sequencing and shotgun metagenomic sequencing

• Published in: Journal of advanced research Vol. 26; pp. 111 - 121
• Main Authors: Han, Dongsheng, Gao, Peng, Li, Rui, Tan, Ping, Xie, Jiehong, Zhang, Rui, Li, Jinming
• Format: Journal Article
• Language: English
• Published: Egypt Elsevier B.V 01.11.2020; Elsevier
• Subjects: 16S rRNA gene sequencing; Microbial community profiling; Microbiome; Microbiota; Shotgun metagenomic sequencing; Shotgun metagenomic sequencing; Microbiota; 16S rRNA gene sequencing; Microbial community profiling; Microbiome

[Display omitted] Microbiome research based on high-throughput sequencing has grown exponentially in recent years, but methodological variations can easily undermine the reproducibility across studies. To systematically evaluate the comparability of sequencing results of 16S rRNA gene sequencing (16...