Formulation, inflammation, and RNA sensing impact the immunogenicity of self-amplifying RNA vaccines

• Published in: Molecular therapy. Nucleic acids Vol. 31; pp. 29 - 42
• Main Authors: Tregoning, John S., Stirling, David C., Wang, Ziyin, Flight, Katie E., Brown, Jonathan C., Blakney, Anna K., McKay, Paul F., Cunliffe, Robert F., Murugaiah, Valarmathy, Fox, Christopher B., Beattie, Mitchell, Tam, Ying K., Johansson, Cecilia, Shattock, Robin J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.03.2023; American Society of Gene & Cell Therapy; Elsevier
• Subjects: formulation; inflammation; influenza; MT: Oligonucleotides: Therapies and Applications; Original; pandemic; sensing; vaccine; influenza; MT: Oligonucleotides: Therapies and Applications; sensing; vaccine; inflammation; formulation; pandemic

To be effective, RNA vaccines require both in situ translation and the induction of an immune response to recruit cells to the site of immunization. These factors can pull in opposite directions with the inflammation reducing expression of the vaccine antigen. We investigated how formulation affects...