Use of formalin-fixed paraffin-embedded tissue and single-strand conformation polymorphism analysis for polymerase chain reaction of antigen receptor rearrangements in dogs

• Published in: Journal of Veterinary Medical Science Vol. 71; no. 4; pp. 535 - 538
• Main Authors: Kaneko, N.(Yamaguchi Univ. (Japan). Faculty of Agriculture), Tanimoto, T, Morimoto, M, Hayashi, T, Shimokawa Miyama, T, Hiraoka, H, Itamoto, K, Une, S, Mizuno, T, Okuda, M
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.04.2009; Japan Science and Technology Agency
• Subjects: Animals; cancer diagnosis; canine; CHIEN; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; Dog Diseases - diagnosis; Dog Diseases - genetics; Dog Diseases - pathology; DOGS; Formaldehyde - pharmacology; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; LEUCEMIA; LEUCEMIE; LEUKAEMIA; LINFOMA; LYMPHOMA; Lymphoma - diagnosis; Lymphoma - genetics; Lymphoma - pathology; Lymphoma - veterinary; lymphoma/leukemia; LYMPHOME; METHODE; METHODS; METODOS; NEOPLASMAS; NEOPLASME; NEOPLASMS; Paraffin Embedding - veterinary; PCR; PERRO; Polymorphism, Single-Stranded Conformational; Retrospective Studies; Tissue Fixation - methods; Tissue Fixation - veterinary; tumor diagnosis

PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples...