Use of formalin-fixed paraffin-embedded tissue and single-strand conformation polymorphism analysis for polymerase chain reaction of antigen receptor rearrangements in dogs

PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples...

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Published in	Journal of Veterinary Medical Science Vol. 71; no. 4; pp. 535 - 538
Main Authors	Kaneko, N.(Yamaguchi Univ. (Japan). Faculty of Agriculture), Tanimoto, T, Morimoto, M, Hayashi, T, Shimokawa Miyama, T, Hiraoka, H, Itamoto, K, Une, S, Mizuno, T, Okuda, M
Format	Journal Article
Language	English
Published	Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.04.2009 Japan Science and Technology Agency
Subjects	Animals cancer diagnosis canine CHIEN DIAGNOSIS DIAGNOSTIC DIAGNOSTICO Dog Diseases - diagnosis Dog Diseases - genetics Dog Diseases - pathology DOGS Formaldehyde - pharmacology Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor LEUCEMIA LEUCEMIE LEUKAEMIA LINFOMA LYMPHOMA Lymphoma - diagnosis Lymphoma - genetics Lymphoma - pathology Lymphoma - veterinary lymphoma/leukemia LYMPHOME METHODE METHODS METODOS NEOPLASMAS NEOPLASME NEOPLASMS Paraffin Embedding - veterinary PCR PERRO Polymorphism, Single-Stranded Conformational Retrospective Studies Tissue Fixation - methods Tissue Fixation - veterinary tumor diagnosis
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Abstract	PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples were shorter than those of the others. Analysis of the formalin fixation time and DNA amplification controls suggested that a formalin fixation time of less than one week is appropriate. Additionally, application of single strand conformation polymorphism (SSCP) for PARR provided clearer results than conventional PARR in 16 unfixed tissues and three FFPE tissues. These results show that PARR-FFPE is viable for tissues with an appropriate formalin fixation time and that application of FFPE and SSCP for PARR are useful for diagnosis and retrospective study of canine lymphoid neoplasia.
AbstractList	PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples were shorter than those of the others. Analysis of the formalin fixation time and DNA amplification controls suggested that a formalin fixation time of less than one week is appropriate. Additionally, application of single strand conformation polymorphism (SSCP) for PARR provided clearer results than conventional PARR in 16 unfixed tissues and three FFPE tissues. These results show that PARR-FFPE is viable for tissues with an appropriate formalin fixation time and that application of FFPE and SSCP for PARR are useful for diagnosis and retrospective study of canine lymphoid neoplasia. PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples were shorter than those of the others. Analysis of the formalin fixation time and DNA amplification controls suggested that a formalin fixation time of less than one week is appropriate. Additionally, application of single strand conformation polymorphism (SSCP) for PARR provided clearer results than conventional PARR in 16 unfixed tissues and three FFPE tissues. These results show that PARR-FFPE is viable for tissues with an appropriate formalin fixation time and that application of FFPE and SSCP for PARR are useful for diagnosis and retrospective study of canine lymphoid neoplasia.PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples were shorter than those of the others. Analysis of the formalin fixation time and DNA amplification controls suggested that a formalin fixation time of less than one week is appropriate. Additionally, application of single strand conformation polymorphism (SSCP) for PARR provided clearer results than conventional PARR in 16 unfixed tissues and three FFPE tissues. These results show that PARR-FFPE is viable for tissues with an appropriate formalin fixation time and that application of FFPE and SSCP for PARR are useful for diagnosis and retrospective study of canine lymphoid neoplasia.
Author	Tanimoto, T Itamoto, K Hiraoka, H Mizuno, T Kaneko, N.(Yamaguchi Univ. (Japan). Faculty of Agriculture) Hayashi, T Shimokawa Miyama, T Une, S Morimoto, M Okuda, M
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Cites_doi	10.1016/S0002-9440(10)65252-2 10.3748/wjg.v10.i7.995 10.1111/j.1476-5810.2005.00080.x 10.1354/vp.43-3-241 10.1136/jcp.47.6.493 10.1016/S1092-9134(00)90014-5 10.1053/hupa.2001.28963 10.1002/cncr.11331 10.1354/vp.40-1-32 10.1073/pnas.86.8.2766 10.1136/jcp.43.11.888
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References	3. Crisi, G.M., Emanuel, J.R., Johnson, C., Crotty, P., Costa, J. and Tallini, G. 2002. Semireannealing, single-stranded conformational polymorphism: a novel and effective tool for the diagnosis of T-cell clonality. Diagn. Mol. Pathol. 11: 67-74. 2. Camilleri-Broet, S., Devez, F., Tissier, F., Ducruit, V., Le Tourneau, A., Diebold, J., Audouin, J. and Molina, T. 2000. Quality control and sensitivity of polymerase chain reaction techniques for the assessment of immunoglobulin heavy chain gene rearrangements from fixed- and paraffin-embedded samples. Ann. Diagn. Pathol. 4: 71-76. 7. Kaneko, N., Okuda, M., Toyama, N., Oikawa, T., Watanabe, M., Kanaya, N., Yazawa, M., Hasegawa, K., Morimoto, M., Hayashi, T., Une, S., Nakaichi, M., Taura, Y., Tsujimoto, H. and Inokuma, H. 2005. Detection of centrosome amplification as a surrogate marker of dysfunction in the p53 pathway -p53 gene mutation or MDM2 overexpression. Vet. Comp. Oncol. 3: 203-210. 10. Signoretti, S., Murphy, M., Cangi, M.G., Puddu, P., Kadin, M.E. and Loda, M. 1999. Detection of clonal T-cell receptor gamma gene rearrangements in paraffin-embedded tissue by polymerase chain reaction and nonradioactive single-strand conformational polymorphism analysis. Am. J. Pathol. 154: 67-75. 12. Vail, D.M. and Young, K.M. 2007. Canine lymphoma and lymphoid leukemias. pp. 600-733. In: Small Animal Clinical Oncology, 4th ed. (Withrow, S.J. and Vail, D.M. eds.), Saunders, Philadelphia. 8. Orba, Y., Tanaka, S., Nishihara, H., Kawamura, N., Itoh, T., Shimizu, M., Sawa, H. and Nagashima, K. 2003. Application of laser capture microdissection to cytologic specimens for the detection of immunoglobulin heavy chain gene rearrangement in patients with malignant lymphoma. Cancer 99: 198-204. 5. Han, X.Q., He, L., Shong, L.Y., Jiang, H.Y., Zhu, M.G. and Zhao, T. 2004. Investigation of T-cell receptor-gamma gene rearrangement in gastrointestinal lymphomas by PCR-SSCP analysis. World J. Gastroenterol. 10: 995-999. 11. Valli, V.E., Vernau, W., de Lorimier, L.P., Graham, P.S. and Moore, P.F. 2006. Canine indolent nodular lymphoma. Vet. Pathol. 43: 241-256. 9. Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K. and Sekiya, T. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. U.S.A. 86: 2766-2770. 1. Burnett, R.C., Vernau, W., Modiano, J.F., Olver, C.S., Moore, P.F. and Avery, A.C. 2003. Diagnosis of canine lymphoid neoplasia using clonal rearrangements of antigen receptor genes. Vet. Pathol. 40: 32-41. 13. Wan, J.H., Trainor, K.J., Brisco, M.J. and Morley, A.A. 1990. Monoclonality in B cell lymphoma detected in paraffin wax embedded sections using the polymerase chain reaction. J. Clin. Pathol. 43: 888-890. 4. Diss, T.C., Pan, L., Peng, H., Wotherspoon, A.C. and Isaacson, P.G. 1994. Sources of DNA for detecting B cell monoclonality using PCR. J. Clin. Pathol. 47: 493-496. 6. Kadin, M.E., Drews, R., Samel, A., Gilchrist, A. and Kocher, O. 2001. Hodgkin's lymphoma of T-cell type: clonal association with a CD30+ cutaneous lymphoma. Hum. Pathol. 32: 1269-1272. 11 12 13 1 2 3 4 5 6 7 8 9 10
References_xml	– reference: 11. Valli, V.E., Vernau, W., de Lorimier, L.P., Graham, P.S. and Moore, P.F. 2006. Canine indolent nodular lymphoma. Vet. Pathol. 43: 241-256. – reference: 2. Camilleri-Broet, S., Devez, F., Tissier, F., Ducruit, V., Le Tourneau, A., Diebold, J., Audouin, J. and Molina, T. 2000. Quality control and sensitivity of polymerase chain reaction techniques for the assessment of immunoglobulin heavy chain gene rearrangements from fixed- and paraffin-embedded samples. Ann. Diagn. Pathol. 4: 71-76. – reference: 5. Han, X.Q., He, L., Shong, L.Y., Jiang, H.Y., Zhu, M.G. and Zhao, T. 2004. Investigation of T-cell receptor-gamma gene rearrangement in gastrointestinal lymphomas by PCR-SSCP analysis. World J. Gastroenterol. 10: 995-999. – reference: 10. Signoretti, S., Murphy, M., Cangi, M.G., Puddu, P., Kadin, M.E. and Loda, M. 1999. Detection of clonal T-cell receptor gamma gene rearrangements in paraffin-embedded tissue by polymerase chain reaction and nonradioactive single-strand conformational polymorphism analysis. Am. J. Pathol. 154: 67-75. – reference: 4. Diss, T.C., Pan, L., Peng, H., Wotherspoon, A.C. and Isaacson, P.G. 1994. Sources of DNA for detecting B cell monoclonality using PCR. J. Clin. Pathol. 47: 493-496. – reference: 3. Crisi, G.M., Emanuel, J.R., Johnson, C., Crotty, P., Costa, J. and Tallini, G. 2002. Semireannealing, single-stranded conformational polymorphism: a novel and effective tool for the diagnosis of T-cell clonality. Diagn. Mol. Pathol. 11: 67-74. – reference: 13. Wan, J.H., Trainor, K.J., Brisco, M.J. and Morley, A.A. 1990. Monoclonality in B cell lymphoma detected in paraffin wax embedded sections using the polymerase chain reaction. J. Clin. Pathol. 43: 888-890. – reference: 9. Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K. and Sekiya, T. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. U.S.A. 86: 2766-2770. – reference: 7. Kaneko, N., Okuda, M., Toyama, N., Oikawa, T., Watanabe, M., Kanaya, N., Yazawa, M., Hasegawa, K., Morimoto, M., Hayashi, T., Une, S., Nakaichi, M., Taura, Y., Tsujimoto, H. and Inokuma, H. 2005. Detection of centrosome amplification as a surrogate marker of dysfunction in the p53 pathway -p53 gene mutation or MDM2 overexpression. Vet. Comp. Oncol. 3: 203-210. – reference: 12. Vail, D.M. and Young, K.M. 2007. Canine lymphoma and lymphoid leukemias. pp. 600-733. In: Small Animal Clinical Oncology, 4th ed. (Withrow, S.J. and Vail, D.M. eds.), Saunders, Philadelphia. – reference: 6. Kadin, M.E., Drews, R., Samel, A., Gilchrist, A. and Kocher, O. 2001. Hodgkin's lymphoma of T-cell type: clonal association with a CD30+ cutaneous lymphoma. Hum. Pathol. 32: 1269-1272. – reference: 8. Orba, Y., Tanaka, S., Nishihara, H., Kawamura, N., Itoh, T., Shimizu, M., Sawa, H. and Nagashima, K. 2003. Application of laser capture microdissection to cytologic specimens for the detection of immunoglobulin heavy chain gene rearrangement in patients with malignant lymphoma. Cancer 99: 198-204. – reference: 1. Burnett, R.C., Vernau, W., Modiano, J.F., Olver, C.S., Moore, P.F. and Avery, A.C. 2003. Diagnosis of canine lymphoid neoplasia using clonal rearrangements of antigen receptor genes. Vet. Pathol. 40: 32-41. – ident: 3 – ident: 10 doi: 10.1016/S0002-9440(10)65252-2 – ident: 5 doi: 10.3748/wjg.v10.i7.995 – ident: 12 – ident: 7 doi: 10.1111/j.1476-5810.2005.00080.x – ident: 11 doi: 10.1354/vp.43-3-241 – ident: 4 doi: 10.1136/jcp.47.6.493 – ident: 2 doi: 10.1016/S1092-9134(00)90014-5 – ident: 6 doi: 10.1053/hupa.2001.28963 – ident: 8 doi: 10.1002/cncr.11331 – ident: 1 doi: 10.1354/vp.40-1-32 – ident: 9 doi: 10.1073/pnas.86.8.2766 – ident: 13 doi: 10.1136/jcp.43.11.888
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SubjectTerms	Animals cancer diagnosis canine CHIEN DIAGNOSIS DIAGNOSTIC DIAGNOSTICO Dog Diseases - diagnosis Dog Diseases - genetics Dog Diseases - pathology DOGS Formaldehyde - pharmacology Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor LEUCEMIA LEUCEMIE LEUKAEMIA LINFOMA LYMPHOMA Lymphoma - diagnosis Lymphoma - genetics Lymphoma - pathology Lymphoma - veterinary lymphoma/leukemia LYMPHOME METHODE METHODS METODOS NEOPLASMAS NEOPLASME NEOPLASMS Paraffin Embedding - veterinary PCR PERRO Polymorphism, Single-Stranded Conformational Retrospective Studies Tissue Fixation - methods Tissue Fixation - veterinary tumor diagnosis
Title	Use of formalin-fixed paraffin-embedded tissue and single-strand conformation polymorphism analysis for polymerase chain reaction of antigen receptor rearrangements in dogs
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