A CRISPR Screen Using Subtilase Cytotoxin Identifies SLC39A9 as a Glycan-Regulating Factor

• Published in: iScience Vol. 15; pp. 407 - 420
• Main Authors: Yamaji, Toshiyuki, Hanamatsu, Hisatoshi, Sekizuka, Tsuyoshi, Kuroda, Makoto, Iwasaki, Norimasa, Ohnishi, Makoto, Furukawa, Jun-ichi, Yahiro, Kinnosuke, Hanada, Kentaro
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 31.05.2019; Elsevier
• Subjects: Biochemistry; Biological Sciences; Cell Biology; Microbiology; Molecular Biology; Biological Sciences; Biochemistry; Molecular Biology; Microbiology; Cell Biology

Subtilase cytotoxin (SubAB) is a virulence factor produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains. The toxin recognizes sialoglycans for entry and cleaves an endoplasmic reticulum chaperon, binding immunoglobulin protein, to cause cell death. However, no...