Tissue-Specific mRNA Expression Profiles of Drug-Metabolizing Enzymes and Transporters in the Cynomolgus Monkey

• Published in: DRUG METABOLISM AND PHARMACOKINETICS Vol. 24; no. 2; pp. 139 - 144
• Main Authors: Nishimura, Masuhiro, Koeda, Akiko, Morikawa, Hiroshi, Satoh, Tetsuo, Narimatsu, Shizuo, Naito, Shinsaku
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2009; Japanese Society for the Study of Xenobiotics
• Subjects: Animals; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Cells, Cultured; cynomolgus monkey; Cytochrome P-450 CYP3A - metabolism; DNA Probes; Dose-Response Relationship, Drug; drug-metabolizing enzymes; Gene Expression; Gene Expression Profiling; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Inactivation, Metabolic; Macaca fascicularis; Male; Membrane Transport Proteins - metabolism; Microsomes, Liver - enzymology; mRNA expression; quantification; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Species Specificity; Tissue Distribution; cynomolgus monkey; quantification; drug-metabolizing enzymes; mRNA expression; tissue distribution

Pairs of forward and reverse primers and TaqMan probes specific to each of 19 drug-metabolizing enzymes (cytochrome P450s, UDP-glucuronosyltransferases, glutathione S-transferases, and sulfotransferases) and 5 transporters (ABC and SLC transporters) in the cynomolgus monkey were prepared. The expres...