Using Gene Editing to Establish a Safeguard System for Pluripotent Stem-Cell-Based Therapies

• Published in: iScience Vol. 22; pp. 409 - 422
• Main Authors: Wu, Youjun, Chang, Tammy, Long, Yan, Huang, He, Kandeel, Fouad, Yee, Jiing-Kuan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.12.2019; Elsevier
• Subjects: Cellular Therapy; Stem Cells Research; Techniques in Genetics; Stem Cells Research; Techniques in Genetics; Cellular Therapy

A major challenge in using human pluripotent stem cells (hPSCs) in therapy is the risk of teratoma formation due to contaminating undifferentiated stem cells. We used CRISPR-Cas9 for in-frame insertion of a suicide gene, iC9, into the endogenous SOX2 locus in human embryonic stem cell (ESC) line H1...