Improved image processing for 3D virtual object construction from serial sections reveals tissue patterns in root tips of Zea mays

• Published in: Applications in plant sciences Vol. 11; no. 6; pp. e11531 - n/a
• Main Authors: Miki, Yasushi, Saito, Susumu, Niki, Teruo, Gladish, Daniel K.
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.11.2023; John Wiley and Sons Inc; Wiley
• Subjects: 3D construction; Application; Batch processing; cell outline extraction; cell structures; Cells; computers; cutting; digital images; extracts; image alignment; image analysis; Image processing; Information processing; Microscopy; Pattern recognition; plant tissues; planting; root tips; Sectioning; Seeds; serial sections; Zea mays; California; United States > US; Japan; Germany; 3D construction; serial sections; image alignment; Zea mays; cell outline extraction

Premise Previously we described methods for generating three‐dimensional (3D) virtual reconstructions of plant tissues from transverse thin sections. Here, we report the applicability of longitudinal sections and improved image‐processing steps that are simpler to perform and utilize free applicatio...