旱半夏组织培养技术的优化研究

以旱半夏无菌苗的叶片、叶柄和块茎为外植体,在不同温度及激素组合的条件下,进行了愈伤组织诱导、芽分化和生根的研究。结果表明：愈伤组织诱导的最适温度为25℃;MS＋KT 1.0 mg/L＋2,4-D 2.0 mg/L对块茎的愈伤诱导效果最好,诱导率达96.7%。温度对芽分化的影响不明显;最佳的芽分化培养基为MS＋KT 3.0 mg/L＋2,4-D 1.0 mg/L。MS＋IBA 0.5 mg/L＋蔗糖30 g/L对单芽增殖效果明显。MS＋KT 1.0 mg/L＋NAA 0.5 mg/L最有利于生根。...

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Bibliographic Details
Published in江西农业学报 Vol. 24; no. 3; pp. 26 - 29
Main Author 王艺霖 屈文国 李昆志
Format Journal Article
LanguageChinese
Published 昆明理工大学,云南昆明,650224 2012
Subjects
分化
半夏
愈伤组织
组织培养
诱导
诱导
分化
组织培养
愈伤组织
半夏
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ISSN1001-8581
DOI10.3969/j.issn.1001-8581.2012.03.007

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Summary:以旱半夏无菌苗的叶片、叶柄和块茎为外植体,在不同温度及激素组合的条件下,进行了愈伤组织诱导、芽分化和生根的研究。结果表明：愈伤组织诱导的最适温度为25℃;MS＋KT 1.0 mg/L＋2,4-D 2.0 mg/L对块茎的愈伤诱导效果最好,诱导率达96.7%。温度对芽分化的影响不明显;最佳的芽分化培养基为MS＋KT 3.0 mg/L＋2,4-D 1.0 mg/L。MS＋IBA 0.5 mg/L＋蔗糖30 g/L对单芽增殖效果明显。MS＋KT 1.0 mg/L＋NAA 0.5 mg/L最有利于生根。
Bibliography:36-1124/S
Using the leaf,leafstalk and rhizome of the aseptic seedlings of Pinellia ternate Thunb.Breit as explants,the author studied the effects of temperature and plant hormones on the inducement of callus,bud differentiation and rooting.The results showed that the optimum temperature for callus inducement was 25 ℃.The optimum medium for the inducement of callus from rhizome was MS＋ KT 1.0 mg/L＋ 2,4-D 2.0 mg/L,and the inducement rate reached 96.7%.Temperature had little effect on the bud differentiation.The optimum medium for bud differentiation was MS＋ KT 3.0 mg/L＋ 2,4-D 1.0 mg/L.The medium MS＋ IBA 0.5 mg/L＋ cane sugar 30 g/L had significant effect on the proliferation of single bud.The medium MS＋ KT 1.0 mg/L＋ NAA 0.5 mg/L was most beneficial for rooting.
WANG Yi-lin,QU Wen-guo,LI Kun-zhi（Kunming University of Science and Technology,Kunming 650224,China）
Pinellia ternate; Tissue culture; Callus; Inducement; Differentiation
ISSN:1001-8581
DOI:10.3969/j.issn.1001-8581.2012.03.007