干细胞转录因子Sox2在肺癌中的表达和意义

背景与目的转录因子Sox2维持干细胞的全能性，参与肿瘤干细胞的自我更新，在多种肿瘤的发生发展中发挥重要作用。本研究旨在探讨Sox2及Sox2自身抗体（Sox2-Ab）在非小细胞肺癌（non-small cell lung cancer, NSCLC）患者组织及血清中的表达和意义。方法荧光定量PCR及免疫组化法检测58例NSCLC、16例其他肿瘤和20例正常肺组织标本中Sox2基因及蛋白表达，ELISA法检测30例NSCLC患者和30例健康体检者血清Sox2-Ab水平，结合NSCLC临床病理特点进行数据分析。结果肺癌组织中Sox2 mRNA水平及蛋白阳性表达率均高于其他肿瘤及正常肺组织，差异均有...

Full description

Saved in:
Bibliographic Details
Published in中国肺癌杂志 Vol. 16; no. 11; pp. 591 - 595
Main Author 许伟 位云艳 谭瑶曦 徐玮 程雁 吴剑卿
Format Journal Article
LanguageChinese
Published 南京医科大学第一附属医院老年呼吸科, 南京,210029%江苏省肿瘤医院病理科,南京,210009%南京医科大学发育遗传学系,南京,210029 2013
Subjects
ELISA法
LC患者
mRNA水平
NSCLC
细胞转录因子
肿瘤干细胞
荧光定量PCR
非小细胞肺癌
Sox2
肺肿瘤
Sox2 self-antibody
Cancer stem cells
Sox2自身抗体
肿瘤干细胞
Lung neoplasms
Online AccessGet full text

Cover

More Information
Summary:背景与目的转录因子Sox2维持干细胞的全能性，参与肿瘤干细胞的自我更新，在多种肿瘤的发生发展中发挥重要作用。本研究旨在探讨Sox2及Sox2自身抗体（Sox2-Ab）在非小细胞肺癌（non-small cell lung cancer, NSCLC）患者组织及血清中的表达和意义。方法荧光定量PCR及免疫组化法检测58例NSCLC、16例其他肿瘤和20例正常肺组织标本中Sox2基因及蛋白表达，ELISA法检测30例NSCLC患者和30例健康体检者血清Sox2-Ab水平，结合NSCLC临床病理特点进行数据分析。结果肺癌组织中Sox2 mRNA水平及蛋白阳性表达率均高于其他肿瘤及正常肺组织，差异均有统计学意义（P〈0.01），且Sox2 mRNA表达增高同肺癌患者病理类型及肿瘤大小有关，与性别、年龄、肿瘤分化程度和淋巴结转移等无关。血清Sox2-Ab水平在NSCLC患者和正常体检者差异无统计学意义。结论Sox2在NSCLC中有较高的表达，与病理类型、肿瘤大小密切相关，Sox2可能成为肺癌新的标志物及治疗靶点。
Bibliography:Wei XU, Yunyan YVEI, Yaoxi TAN, Wei XU, Steven Yan CHENG, Jianqing WU （1 Department of Geriatrics, the First Affilicated Hospital of Nanjing Medical University, Nanjing 210029, China; 2 Department of Pathologyj Jzangsu Cancer Hospttalj Nanjmg 210029, China; 3 Department of Developmental Geneticsj Nanlmg Medical Universityj Nanjing 210029, China）
Background and objective Transcription factor Sox2 remains the pluripotency of stem cell, partici-pates in self-renew of cancer stem cell and plays important role during the initiation and development of various cancers. hTis study intends to investigate the expression and signiifcance of Sox2 and Sox2 autoantibodies （Sox2-Ab） in tissue and serum of patients with non-small cell lung cancer （NSCLC）. Methods Expression of Sox2 gene and protein was tested in 58 cases of NSCLC, 16 patients with other tumors and 20 cases of normal lung tissue specimens by quantitative PCR and immunohis-tochemical assay, respectively. Serum Sox2-Ab level was detected in 30 cases of NSCLC pat
ISSN:1009-3419
1999-6187
DOI:10.3779/j.issn.1009-3419.2013.11.05