Molecular Cloning of an Essential Subunit of RNA Polymerase II Elongation Factor SIII

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 12; pp. 5237 - 5241
• Main Authors: Garrett, Karla Pfeil, Tan, Siyuan, Bradsher, John N., Lane, William S., Conaway, Joan Weliky, Conaway, Ronald C.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 07.06.1994; National Acad Sciences; National Academy of Sciences
• Subjects: Amino Acid Sequence; Amino acids; Animals; Base Sequence; Biochemistry; Cloning, Molecular; Complementary DNA; DNA Primers - chemistry; DNA, Complementary - genetics; Generally accepted auditing standards; Genes; Liver; Molecular cloning; Molecular Sequence Data; Peptide elongation factors; Polymerase chain reaction; Protein Structure, Secondary; Rats; Rho Factor - chemistry; Ribonucleic acid; RNA; RNA Polymerase II - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Transcription factors; Transcription Factors - genetics

A transcription factor designated SIII was recently purified from mammalian cells and shown to regulate the activity of the RNA polymerase II elongation complex. SIII is a heterotrimer composed of ≈110-, 18-, and 15-kDa polypeptides and is capable of increasing the overall rate of RNA chain elongati...