Antiphotoaging and Skin-Protective Activities of Ardisia silvestris Ethanol Extract in Human Keratinocytes

• Published in: Plants (Basel) Vol. 12; no. 5; p. 1167
• Main Authors: Huang, Lei, You, Long, Aziz, Nur, Yu, Seung Hui, Lee, Jong Sub, Choung, Eui Su, Luong, Van Dung, Jeon, Mi-Jeong, Hur, Moonsuk, Lee, Sarah, Lee, Byoung-Hee, Kim, Han Gyung, Cho, Jae Youl
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.03.2023; MDPI
• Subjects: Activator protein 1; Aging (artificial); anti-apoptosis; antioxidative capacity; AP-1; Apoptosis; Ardisia; Ardisia silvestris ethanol extract; Assaying; c-Jun protein; Care and treatment; Cell death; Cosmetics; Cytotoxicity; Dermatology; Ethanol; Genetic aspects; Herbal medicine; High performance liquid chromatography; Hyaluronic acid; Immunoblotting; JNK protein; Keratinocytes; Kinases; Liquid chromatography; MAP kinase; Materia medica, Vegetable; Medicinal plants; Mitogen-activated protein kinases; Oxidation; Physiological aspects; Plant extracts; Protected species; Proteins; Reactive oxygen species; Reporter gene; ROS; Rutin; Scavenging; Signal transduction; Signaling; Skin; Toxicity; Transcription factors; Ultraviolet radiation; UVB irradiation; South Korea; Vietnam; antioxidative capacity; anti-apoptosis; AP-1; Ardisia silvestris ethanol extract; ROS; UVB irradiation
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12051167

is a traditional medicinal herb used in Vietnam and several other countries. However, the skin-protective properties of ethanol extract (As-EE) have not been evaluated. Human keratinocytes form the outermost barrier of the skin and are the main target of ultraviolet (UV) radiation. UV exposure causes skin photoaging via the production of reactive oxygen species. Protection from photoaging is thus a key component of dermatological and cosmetic products. In this research, we found that As-EE can prevent UV-induced skin aging and cell death as well as enhance the barrier effect of the skin. First, the radical-scavenging ability of As-EE was checked using DPPH, ABTS, TPC, CUPRAC, and FRAP assays, and a 3-(4-5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay was used to examine cytotoxicity. Reporter gene assays were used to determine the doses that affect skin-barrier-related genes. A luciferase assay was used to identify possible transcription factors. The anti-photoaging mechanism of As-EE was investigated by determining correlated signaling pathways using immunoblotting analyses. As-EE had no harmful effects on HaCaT cells, according to our findings, and As-EE revealed moderate radical-scavenging ability. With high-performance liquid chromatography (HPLC) analysis, rutin was found to be one of the major components. In addition, As-EE enhanced the expression levels of hyaluronic acid synthase-1 and occludin in HaCaT cells. Moreover, As-EE dose-dependently up-regulated the production of occludin and transglutaminase-1 after suppression caused by UVB blocking the activator protein-1 signaling pathway, in particular, the extracellular response kinase and c-Jun N-terminal kinase. Our findings suggest that As-EE may have anti-photoaging effects by regulating mitogen-activated protein kinase, which is good news for the cosmetics and dermatology sectors.