Structural basis for inhibition of the SARS-CoV-2 RNA polymerase by suramin

• Published in: Nature structural & molecular biology Vol. 28; no. 3; pp. 319 - 325
• Main Authors: Yin, Wanchao, Luan, Xiaodong, Li, Zhihai, Zhou, Ziwei, Wang, Qingxing, Gao, Minqi, Wang, Xiaoxi, Zhou, Fulai, Shi, Jingjing, You, Erli, Liu, Mingliang, Wang, Qingxia, Jiang, Yi, Jiang, Hualiang, Xiao, Gengfu, Zhang, Leike, Yu, Xuekui, Zhang, Shuyang, Eric Xu, H.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2021; Nature Publishing Group
• Subjects: 101/28; 631/154; 631/535/1258; 82/80; 82/83; Animals; Antiparasitic agents; Antiviral Agents - chemistry; Antiviral Agents - metabolism; Antiviral Agents - pharmacology; Binding Sites; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Catalytic Domain; Chemical properties; Chlorocebus aethiops; Coronavirus RNA-Dependent RNA Polymerase - antagonists & inhibitors; Coronavirus RNA-Dependent RNA Polymerase - chemistry; Coronavirus RNA-Dependent RNA Polymerase - metabolism; Coronaviruses; COVID-19; Cryoelectron Microscopy; DNA-directed RNA polymerase; Electron microscopy; Enzyme binding; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - metabolism; Enzyme Inhibitors - pharmacology; Health aspects; Inhibitors; Life Sciences; Membrane Biology; Nucleotides; Pandemics; Pharmacology, Experimental; Protein Conformation; Protein Structure; RNA polymerase; RNA polymerases; RNA, Viral - chemistry; RNA, Viral - metabolism; RNA-directed RNA polymerase; SARS-CoV-2 - drug effects; Severe acute respiratory syndrome coronavirus 2; Structure; Suramin; Suramin - chemistry; Suramin - metabolism; Suramin - pharmacology; Suramin sodium; Vero Cells; Viral diseases; Virus Replication - drug effects; China

The COVID-19 pandemic caused by nonstop infections of SARS-CoV-2 has continued to ravage many countries worldwide. Here we report that suramin, a 100-year-old drug, is a potent inhibitor of the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) and acts by blocking the binding of RNA to the enzyme. In b...