Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope

• Published in: Quantitative plant biology Vol. 3; p. e15
• Main Authors: Shimadzu, Shunji, Furuya, Tomoyuki, Ozawa, Yasuko, Fukuda, Hiroo, Kondo, Yuki
• Format: Journal Article
• Language: English
• Published: England Cambridge University Press 18.07.2022
• Subjects: Biological activity; Biological analysis; Biomarkers; Cameras; Cell differentiation; Cell fate; Cloning; Excitation; Fluorescence; Gene expression; Imaging; Kinases; Luminescence; luminescence imaging; Methods; Monitoring; Original; Photobleaching; Plant cells; Proteins; Spatial analysis; spatio-temporal dynamics; Stem cells; vascular development; Xylem; luminescence imaging; vascular development; spatio-temporal dynamics

Stem cell fates are spatio-temporally regulated during plant development. Time-lapse imaging of fluorescence reporters is the most widely used method for spatio-temporal analysis of biological processes. However, excitation light for imaging fluorescence reporters causes autofluorescence and photobl...