A sensitive and rapid ultra HPLC–MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 880; no. 1; pp. 132 - 139
• Main Authors: Peer, Cody J., Spencer, Shawn D., VanDenBerg, Dustin A.H., Pacanowski, Michael A., Horenstein, Richard B., Figg, William D.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.2012; Elsevier
• Subjects: Active metabolite; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Blood; Chromatography; Chromatography, High Pressure Liquid - methods; Clopidogrel; derivatization; Drug Stability; Elution; Fundamental and applied biological sciences. Psychology; General pharmacology; Human; Humans; Ionization; liquid chromatography; Materials handling; Medical sciences; Metabolites; pharmacokinetics; Pharmacology. Drug treatments; Reproducibility of Results; Run time (computers); Sensitivity and Specificity; Sulfhydryl Compounds - blood; Sulfhydryl Compounds - pharmacokinetics; tandem mass spectrometry; Tandem Mass Spectrometry - methods; thiols; Ticlopidine - analogs & derivatives; Ticlopidine - blood; Ticlopidine - chemistry; Ticlopidine - pharmacokinetics; Ultra HPLC–MS/MS; LLOQ; QC; uHPLC; MS; Clopidogrel; Active metabolite; Ultra HPLC–MS/MS; CAMD; MS/MS; EDTA; MPB; Human; Ultra performance liquid chromatography; Biological fluid; Thiol; Metabolite; Ultra HPLC-MS/MS; HPLC chromatography; Derivatization; Antiplatelet agent; Blood plasma; Simultaneous measurement; Antithrombotic agent; Thienopyridine derivative

► We developed and validated a novel uHPLC–MS/MS assay for the simultaneous quantification of clopidogrel and its derivatized active metabolite. ► Clinically relevant calibration ranges for clopidogrel (0.01–50ng/mL) and active metabolite (0.1–150ng/m) were used. ► Successfully applied to clinical p...