微生物溶菌酶微胶囊制备方法及其特性研究

利用锐孔法包被一种微生物溶菌酶,探讨单一海藻酸钠为壁材制备微胶囊的最佳工艺参数,比较添加与不添加辅助壁材卡拉胶或壳聚糖,以及利用一步法与二步法静电络合壳聚糖制得的微胶囊的强度性能、载酶量和酶活包埋率.结果确定最佳工艺参数为：海藻酸钠浓度2.0％,微生物溶菌酶浓度0.2％,氯化钙浓度2.0％,固定化时间1h.添加辅助壁材卡拉胶或壳聚糖均有利于增加微胶囊的机械强度及载酶量,添加卡拉胶比不添加卡拉胶制得的微胶囊抑菌效果无明显差异.壳聚糖一步法酶活包埋率最高,两步法最低....

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Published in广东农业科学 Vol. 40; no. 19; pp. 101 - 104
Main Author 李晓晖 费国琴 宋娟 李文莉 宁喜斌
Format Journal Article
LanguageChinese
Published 上海海洋大学食品学院/上海水产品加工及贮藏工程技术研究中心,上海,201306 2013
Subjects
工艺参数
强度性能
微生物溶菌酶
微胶囊
载酶量
酶活包埋率
工艺参数
载酶量
微生物溶菌酶
强度性能
酶活包埋率
微胶囊
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ISSN1004-874X

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Summary:利用锐孔法包被一种微生物溶菌酶,探讨单一海藻酸钠为壁材制备微胶囊的最佳工艺参数,比较添加与不添加辅助壁材卡拉胶或壳聚糖,以及利用一步法与二步法静电络合壳聚糖制得的微胶囊的强度性能、载酶量和酶活包埋率.结果确定最佳工艺参数为：海藻酸钠浓度2.0％,微生物溶菌酶浓度0.2％,氯化钙浓度2.0％,固定化时间1h.添加辅助壁材卡拉胶或壳聚糖均有利于增加微胶囊的机械强度及载酶量,添加卡拉胶比不添加卡拉胶制得的微胶囊抑菌效果无明显差异.壳聚糖一步法酶活包埋率最高,两步法最低.
Bibliography:44-1267/S
LI Xiao-hui, FEI Guo-qin, SONG Juan, LI Wen-li, NING Xi-bing （ Colledge of Food Science and Technology, Shanghai Ocean UniversityShanghai Engineering Research Center of Aquatic-Product Processing ＆ Preservation, shanghai 201306, China）
On this research, a microcapsule of microbial lysozyme was prepared by extrusion method. At first, the parameters of microcapsule preparation with single alginate wall material was optimalized. Then were compared by the strength properties, enzyme loading and enzyme activity embedding rate of the microcapsules adding carrageenan or chitosan as secondary wall material. According to the results, the alginate-chitosan microcapsule was prepared by the electrostatic complexation principle and divided into one-step method and two-step method. The optimum process parameters were as follows： the concentration of sodium alginate was 2.0%; the concentration of microbial lysozyme was 0.2%; the concentration of calcium chloride was 2.0% and the immobilization time was lh. In additi
ISSN:1004-874X