Kinetic Analysis of Estrogen Receptor/Ligand Interactions

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 99; no. 13; pp. 8562 - 8567
• Main Authors: Rich, Rebecca L., Hoth, Lise R., Geoghegan, Kieran F., Brown, Thomas A., LeMotte, Peter K., Simons, Samuel P., Hensley, Preston, Myszka, David G.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 25.06.2002; National Acad Sciences
• Subjects: Agonists; Analysis; Antibodies; Antibodies, Monoclonal - metabolism; Benzhydryl Compounds; Biochemistry; Biological Sciences; Biosensing techniques; Bisphenols; Dehydroepiandrosterone - metabolism; Drug interactions; Estrogen Receptor alpha; Estrogen Receptor beta; Estrogens; Kinetics; Ligands; Phenols - metabolism; Protein isoforms; Receptors; Receptors, Estrogen - chemistry; Receptors, Estrogen - metabolism; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism

Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from nonligands. In a high-resolution mode, the...