Kinetic Analysis of Estrogen Receptor/Ligand Interactions
Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from nonligands. In a high-resolution mode, the...
Saved in:
| Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 99; no. 13; pp. 8562 - 8567 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
National Academy of Sciences
25.06.2002
National Acad Sciences |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from nonligands. In a high-resolution mode, the association and dissociation phase binding responses were shown to be reproducible and could be fit globally to a simple interaction model to extract reaction rate constants. On average, antagonist ligands (such as tamoxifen and nafoxidine) were observed to bind to the receptor with association rates that were 500-fold slower than agonists (such as estriol and β-estradiol). This finding is consistent with these antagonists binding to an altered conformation of the receptor. The biosensor assay also could identify subtle differences in how the same ligand interacted with two different isoforms of the receptor (α and β). The biosensor's ability to determine kinetic rate constants for small molecule/protein interactions provides unique opportunities to understand the mechanisms associated with complex formation as well as new information to drive the optimization of drug candidates. |
|---|---|
| AbstractList | Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from nonligands. In a high-resolution mode, the association and dissociation phase binding responses were shown to be reproducible and could be fit globally to a simple interaction model to extract reaction rate constants. On average, antagonist ligands (such as tamoxifen and nafoxidine) were observed to bind to the receptor with association rates that were 500-fold slower than agonists (such as estriol and β-estradiol). This finding is consistent with these antagonists binding to an altered conformation of the receptor. The biosensor assay also could identify subtle differences in how the same ligand interacted with two different isoforms of the receptor (α and β). The biosensor's ability to determine kinetic rate constants for small molecule/protein interactions provides unique opportunities to understand the mechanisms associated with complex formation as well as new information to drive the optimization of drug candidates. Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from nonligands. In a high-resolution mode, the association and dissociation phase binding responses were shown to be reproducible and could be fit globally to a simple interaction model to extract reaction rate constants. On average, antagonist ligands (such as tamoxifen and nafoxidine) were observed to bind to the receptor with association rates that were 500-fold slower than agonists (such as estriol and beta-estradiol). This finding is consistent with these antagonists binding to an altered conformation of the receptor. The biosensor assay also could identify subtle differences in how the same ligand interacted with two different isoforms of the receptor (alpha and beta). The biosensor's ability to determine kinetic rate constants for small molecule/protein interactions provides unique opportunities to understand the mechanisms associated with complex formation as well as new information to drive the optimization of drug candidates. Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human estrogen receptor. In a screening mode, specific ligands of the receptor were easily discerned from noligands. |
| Author | Geoghegan, Kieran F. Rich, Rebecca L. Simons, Samuel P. Hoth, Lise R. LeMotte, Peter K. Hensley, Preston Myszka, David G. Brown, Thomas A. |
| AuthorAffiliation | Center for Biomolecular Interaction Analysis, University of Utah, Salt Lake City, UT 84132; and † Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340 |
| AuthorAffiliation_xml | – name: Center for Biomolecular Interaction Analysis, University of Utah, Salt Lake City, UT 84132; and † Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340 |
| Author_xml | – sequence: 1 givenname: Rebecca L. surname: Rich fullname: Rich, Rebecca L. – sequence: 2 givenname: Lise R. surname: Hoth fullname: Hoth, Lise R. – sequence: 3 givenname: Kieran F. surname: Geoghegan fullname: Geoghegan, Kieran F. – sequence: 4 givenname: Thomas A. surname: Brown fullname: Brown, Thomas A. – sequence: 5 givenname: Peter K. surname: LeMotte fullname: LeMotte, Peter K. – sequence: 6 givenname: Samuel P. surname: Simons fullname: Simons, Samuel P. – sequence: 7 givenname: Preston surname: Hensley fullname: Hensley, Preston – sequence: 8 givenname: David G. surname: Myszka fullname: Myszka, David G. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/12077320$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkUFv1DAQRi1URLcLV04IRRzglO2M7ST2oYeqKlCxEhKCs-U4zuJV1l5sB9F_T6JdlsIBTnOY941m5l2QMx-8JeQ5wgqhYZd7r9MKOaVCoJSPyAJBYllzCWdkAUCbUnDKz8lFSlsAkJWAJ-QcKTQNo7Ag8oPzNjtTXHs93CeXitAXtynHsLG--GSN3ecQL9duo31X3PlsozbZBZ-ekse9HpJ9dqxL8uXt7eeb9-X647u7m-t1aSpR5dJq1hiOvDdUys5KzXRbA-26tkNremqF6Exfg-QggNtKCmiNbIzsdE95C2xJrg5z92O7s52xPkc9qH10Ox3vVdBO_dnx7qvahO8KKWeIU_71MR_Dt9GmrHYuGTsM2tswJtWgqKmk9L8gCo6spvNGr_4Ct2GM0_-SooBMUpyeuySrA2RiSCna_rQxgprVqVmdOqmbAi8f3vkbP7p6AMzBX20pFTIlqno-4M0_AdWPw5DtjzyRLw7kNk12TyiDSkJVsZ_0M7hV |
| CitedBy_id | crossref_primary_10_1016_j_scitotenv_2018_03_003 crossref_primary_10_1016_j_biochi_2022_02_008 crossref_primary_10_1002_cbic_201100617 crossref_primary_10_1016_j_bcp_2007_12_012 crossref_primary_10_3389_fchem_2020_00728 crossref_primary_10_1155_2013_501851 crossref_primary_10_1016_j_jtbi_2018_02_009 crossref_primary_10_1529_biophysj_105_077818 crossref_primary_10_1016_j_jmb_2022_167464 crossref_primary_10_2165_00024677_200605060_00005 crossref_primary_10_1007_s10549_019_05435_1 crossref_primary_10_1016_j_anndiagpath_2018_02_007 crossref_primary_10_1134_S0012496614010050 crossref_primary_10_1021_jm4011737 crossref_primary_10_1021_acs_jcim_9b00360 crossref_primary_10_1371_journal_pone_0030397 crossref_primary_10_1016_j_procbio_2014_01_021 crossref_primary_10_2217_nnm_11_102 crossref_primary_10_1002_anie_201410148 crossref_primary_10_1016_j_omto_2021_02_014 crossref_primary_10_3390_s150510481 crossref_primary_10_3810_pgm_2009_01_1949 crossref_primary_10_1002_etc_2939 crossref_primary_10_1021_bc9002212 crossref_primary_10_1073_pnas_2216906120 crossref_primary_10_1074_jbc_M111_294843 crossref_primary_10_1007_s10549_017_4243_3 crossref_primary_10_1021_cn1000205 crossref_primary_10_1007_s00216_004_2952_z crossref_primary_10_1177_1933719112462630 crossref_primary_10_1007_s12199_010_0146_1 crossref_primary_10_1016_j_cellsig_2007_05_012 crossref_primary_10_1093_carcin_bgz176 crossref_primary_10_1016_j_aca_2021_338625 crossref_primary_10_1002_jmr_972 crossref_primary_10_1038_nrendo_2017_42 crossref_primary_10_1016_j_jpba_2014_03_002 crossref_primary_10_15252_embr_202255326 crossref_primary_10_1007_s12274_017_1591_6 crossref_primary_10_1073_pnas_0409206102 crossref_primary_10_1016_j_bbrc_2022_05_098 crossref_primary_10_1126_scisignal_aad8170 crossref_primary_10_1093_toxsci_kfs288 crossref_primary_10_1007_s00216_006_0958_4 crossref_primary_10_1007_s10719_007_9076_6 crossref_primary_10_1016_j_cmpb_2012_04_010 crossref_primary_10_1093_toxsci_kfx177 crossref_primary_10_1074_jbc_M411565200 crossref_primary_10_1016_j_tox_2013_04_001 crossref_primary_10_1039_b507237a crossref_primary_10_2139_ssrn_4120108 crossref_primary_10_1021_mp800206b crossref_primary_10_3390_genes12121846 crossref_primary_10_1021_ac1002245 crossref_primary_10_1002_pmic_200401032 crossref_primary_10_1016_j_steroids_2015_01_015 crossref_primary_10_1186_1477_7827_12_66 crossref_primary_10_1021_acs_jmedchem_7b00682 crossref_primary_10_1021_bi900258v crossref_primary_10_1158_0008_5472_CAN_15_2357 crossref_primary_10_1016_j_trac_2023_117079 crossref_primary_10_1039_B612492E crossref_primary_10_1016_j_bios_2014_11_025 crossref_primary_10_1038_srep27658 crossref_primary_10_3390_ijerph120302612 crossref_primary_10_1021_cb800004s crossref_primary_10_1002_jmr_744 crossref_primary_10_1016_j_ijpharm_2006_02_047 crossref_primary_10_1074_jbc_M113_521708 crossref_primary_10_1016_j_ab_2018_06_027 crossref_primary_10_1016_j_abb_2013_11_006 crossref_primary_10_1080_10408398_2010_500499 crossref_primary_10_1002_ange_202101261 crossref_primary_10_1007_s00249_006_0070_x crossref_primary_10_1016_j_molcatb_2003_12_024 crossref_primary_10_1021_es5002659 crossref_primary_10_3389_fpls_2015_00131 crossref_primary_10_1089_jir_2012_0160 crossref_primary_10_1002_ange_201410148 crossref_primary_10_1155_2016_9081375 crossref_primary_10_1177_1087057106297567 crossref_primary_10_1007_s11596_010_0108_2 crossref_primary_10_1016_j_optcom_2010_06_010 crossref_primary_10_1038_nchembio775 crossref_primary_10_3390_s23104831 crossref_primary_10_1002_cem_1010 crossref_primary_10_1002_cyto_a_20279 crossref_primary_10_1002_anie_202101261 crossref_primary_10_1016_j_tibtech_2005_05_002 crossref_primary_10_1007_s11082_023_04703_1 crossref_primary_10_1016_j_chroma_2008_07_089 crossref_primary_10_1007_s10812_010_9389_2 crossref_primary_10_1007_s00216_011_4720_1 crossref_primary_10_1016_j_bcp_2006_09_023 crossref_primary_10_1039_c3lc41330f crossref_primary_10_1002_prot_22503 crossref_primary_10_3390_biomedicines9060681 crossref_primary_10_1016_j_optcom_2017_11_039 crossref_primary_10_1021_bm301394w crossref_primary_10_1016_j_copbio_2006_06_012 crossref_primary_10_1016_j_jsbmb_2015_01_007 crossref_primary_10_1002_jmr_649 crossref_primary_10_1080_1062936X_2011_569899 crossref_primary_10_1186_s12915_022_01293_4 crossref_primary_10_1007_s13233_016_4019_3 crossref_primary_10_1007_s11426_011_4337_4 crossref_primary_10_1016_j_electacta_2013_02_085 crossref_primary_10_1016_j_biopha_2021_112274 crossref_primary_10_1021_es049681e crossref_primary_10_1046_j_1432_1033_2003_03937_x crossref_primary_10_1002_0471140864_ps1913s45 crossref_primary_10_1088_1478_3975_13_6_066011 crossref_primary_10_1039_c3em00023k crossref_primary_10_1039_C5CP00327J crossref_primary_10_3390_s23156703 crossref_primary_10_1016_j_aquatox_2017_02_010 crossref_primary_10_1021_bc800378a crossref_primary_10_1021_bi900522y crossref_primary_10_1021_jp407057f crossref_primary_10_1155_2016_6070742 crossref_primary_10_3390_bios8010001 crossref_primary_10_1039_c2ay25780g crossref_primary_10_1016_j_steroids_2006_03_004 crossref_primary_10_1039_D3CS00709J crossref_primary_10_1177_2470289718801379 crossref_primary_10_1016_j_mce_2016_11_019 crossref_primary_10_1016_j_medengphy_2023_103982 crossref_primary_10_1016_j_scitotenv_2019_134140 crossref_primary_10_1186_1752_0509_6_138 crossref_primary_10_1517_17460441_1_5_439 crossref_primary_10_1080_09513590600927017 crossref_primary_10_1002_jmr_660 crossref_primary_10_1007_s00216_016_9967_0 crossref_primary_10_1080_26896583_2020_1761204 crossref_primary_10_1016_j_bios_2009_02_019 crossref_primary_10_1897_06_482R_1 crossref_primary_10_1016_j_trac_2013_08_012 crossref_primary_10_1021_bc300664k crossref_primary_10_1002_rmv_1952 crossref_primary_10_1177_1087057106296496 crossref_primary_10_1007_s00216_007_1725_x crossref_primary_10_1021_tx400182n crossref_primary_10_1016_j_bbamem_2005_03_014 crossref_primary_10_1529_biophysj_106_082917 crossref_primary_10_1016_j_jsbmb_2005_04_030 crossref_primary_10_1016_j_chemosphere_2018_03_009 crossref_primary_10_1007_s11427_017_9270_x crossref_primary_10_1016_S0006_291X_02_02830_9 crossref_primary_10_1515_BC_2003_053 crossref_primary_10_1002_pmic_200300610 crossref_primary_10_2131_fts_3_33 crossref_primary_10_1021_es304030x crossref_primary_10_1142_S108842461950161X crossref_primary_10_1590_S1516_31802009000300004 crossref_primary_10_1089_adt_2010_0345 crossref_primary_10_1007_s10822_017_0086_6 crossref_primary_10_1016_j_chemosphere_2007_04_047 crossref_primary_10_1016_j_coemr_2019_04_008 crossref_primary_10_1016_j_optcom_2020_125337 crossref_primary_10_3389_fphar_2022_879020 |
| Cites_doi | 10.1016/S1461-5347(00)00288-1 10.1074/jbc.M006777200 10.1177/108705710000500204 10.1177/108705710100600503 10.1006/prep.2001.1409 10.1021/jm980048b 10.1093/toxsci/54.1.138 10.1016/S0092-8674(00)81717-1 10.1021/jm9606352 10.1210/endo.138.3.4979 10.1006/gcen.2000.7521 10.1038/39645 10.1210/endo.139.10.6216 10.1021/bi010379h 10.1006/abio.2001.5314 10.1002/(SICI)1099-1352(199909/10)12:5<279::AID-JMR473>3.0.CO;2-3 10.1210/endo.139.4.5902 10.1073/pnas.95.11.5998 10.1677/joe.0.1700137 10.1016/S1074-5521(01)00025-4 10.1016/S0006-3495(98)77549-6 10.1210/endo.140.2.6480 10.1073/pnas.97.3.1008 10.1006/abio.1998.2990 10.1124/mol.54.1.105 10.1016/0022-4731(85)90049-4 10.1016/S0958-1669(99)00054-3 10.1110/ps.4330102 10.1016/S0968-0004(98)01183-9 10.1074/jbc.M011651200 10.1016/0022-4731(89)90206-9 10.1016/S0960-0760(99)00065-5 10.1021/ci980140g 10.1074/jbc.M009870200 10.1021/jm990078u 10.1021/bi971746l 10.1021/jm991174y 10.1016/S0006-2952(01)00731-6 10.1289/ehp.98106551 |
| ContentType | Journal Article |
| Copyright | Copyright 1993-2002 National Academy of Sciences of the United States of America Copyright National Academy of Sciences Jun 25, 2002 Copyright © 2002, The National Academy of Sciences 2002 |
| Copyright_xml | – notice: Copyright 1993-2002 National Academy of Sciences of the United States of America – notice: Copyright National Academy of Sciences Jun 25, 2002 – notice: Copyright © 2002, The National Academy of Sciences 2002 |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7QO 7X8 5PM |
| DOI | 10.1073/pnas.142288199 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts Biotechnology Research Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Virology and AIDS Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database Nucleic Acids Abstracts Ecology Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Entomology Abstracts Genetics Abstracts Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Immunology Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts Biotechnology Research Abstracts MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE Virology and AIDS Abstracts Engineering Research Database MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) |
| EISSN | 1091-6490 |
| EndPage | 8567 |
| ExternalDocumentID | 144960261 10_1073_pnas_142288199 12077320 99_13_8562 3059055 |
| Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, U.S. Gov't, P.H.S Journal Article Feature |
| GrantInformation_xml | – fundername: NIDDK NIH HHS grantid: F32 DK010150 – fundername: NIDDK NIH HHS grantid: F32 DK10150 |
| GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 123 29P 2AX 2FS 2WC 3O- 4.4 53G 5RE 5VS 85S AACGO AAFWJ AANCE AAYJJ ABBHK ABOCM ABPLY ABPPZ ABTLG ABXSQ ABZEH ACGOD ACIWK ACNCT ACPRK ADACV ADULT AENEX AEUPB AEXZC AFFNX AFOSN AFRAH ALMA_UNASSIGNED_HOLDINGS AQVQM AS~ BKOMP CS3 D0L DCCCD DIK DOOOF DU5 E3Z EBS EJD F5P FRP GX1 H13 HGD HH5 HQ3 HTVGU HYE IPSME JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST KQ8 L7B LU7 MVM N9A NEJ N~3 O9- OK1 P-O PNE PQQKQ R.V RHF RHI RNA RNS RPM RXW SA0 SJN TAE TN5 UKR VOH VQA W8F WH7 WHG WOQ WOW X7M XSW Y6R YBH YKV YSK ZCA ZCG ~02 ~KM - 02 08R 0R 1AW 55 AAPBV ABFLS ABPTK ADACO ADZLD AFDAS AJYGW AS ASUFR DNJUQ DWIUU DZ F20 GJ KM OHM PQEST X XFK XHC ZA5 CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7QO 7X8 5PM |
| ID | FETCH-LOGICAL-c585t-ea37c414fc299de9a3ab602ddbd1ecf2e88dcf60940804e5980bc97c9daf24b03 |
| IEDL.DBID | RPM |
| ISSN | 0027-8424 |
| IngestDate | Tue Sep 17 21:05:26 EDT 2024 Sat Oct 26 00:28:08 EDT 2024 Fri Oct 25 09:50:10 EDT 2024 Tue Nov 19 03:44:39 EST 2024 Fri Dec 06 04:29:23 EST 2024 Sat Sep 28 07:36:36 EDT 2024 Wed Nov 11 00:29:34 EST 2020 Thu May 30 08:50:53 EDT 2019 Tue Dec 10 23:06:17 EST 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 13 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c585t-ea37c414fc299de9a3ab602ddbd1ecf2e88dcf60940804e5980bc97c9daf24b03 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Communicated by Pamela J. Bjorkman, California Institute of Technology, Pasadena, CA To whom reprint requests should be addressed at: Center for Biomolecular Interaction Analysis, School of Medicine 4A417, 50 North Medical Drive, Salt Lake City, UT. E-mail: dmyszka@cores.utah.edu. |
| OpenAccessLink | https://europepmc.org/articles/pmc124311?pdf=render |
| PMID | 12077320 |
| PQID | 201392177 |
| PQPubID | 42026 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_18413620 proquest_journals_201392177 pubmed_primary_12077320 jstor_primary_3059055 pubmedcentral_primary_oai_pubmedcentral_nih_gov_124311 crossref_primary_10_1073_pnas_142288199 pnas_primary_99_13_8562_fulltext proquest_miscellaneous_71862922 pnas_primary_99_13_8562 |
| ProviderPackageCode | RNA PNE |
| PublicationCentury | 2000 |
| PublicationDate | 2002-06-25 |
| PublicationDateYYYYMMDD | 2002-06-25 |
| PublicationDate_xml | – month: 06 year: 2002 text: 2002-06-25 day: 25 |
| PublicationDecade | 2000 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Washington |
| PublicationTitle | Proceedings of the National Academy of Sciences - PNAS |
| PublicationTitleAlternate | Proc Natl Acad Sci U S A |
| PublicationYear | 2002 |
| Publisher | National Academy of Sciences National Acad Sciences |
| Publisher_xml | – sequence: 0 name: National Academy of Sciences – name: National Acad Sciences – name: National Academy of Sciences |
| References | 9398213 - Biochemistry. 1997 Dec 2;36(48):14897-905 9528995 - Endocrinology. 1998 Apr;139(4):2068-76 9875847 - Cell. 1998 Dec 23;95(7):927-37 10094611 - J Chem Inf Comput Sci. 1999 Jan-Feb;39(1):164-8 10803607 - J Biomol Screen. 2000 Apr;5(2):77-88 11967359 - Protein Sci. 2002 May;11(5):1017-25 9721254 - Environ Health Perspect. 1998 Sep;106(9):551-7 11689129 - J Biomol Screen. 2001 Oct;6(5):291-303 9600906 - Proc Natl Acad Sci U S A. 1998 May 26;95(11):5998-6003 11554715 - Anal Biochem. 2001 Sep 15;296(2):197-207 9003514 - J Med Chem. 1997 Jan 17;40(2):146-67 11278577 - J Biol Chem. 2001 May 4;276(18):15059-65 9338790 - Nature. 1997 Oct 16;389(6652):753-8 10746941 - Toxicol Sci. 2000 Mar;54(1):138-53 11303023 - J Biol Chem. 2001 Jun 29;276(26):23397-404 11394045 - Ernst Schering Res Found Workshop. 2001;(34):181-201 4094420 - J Steroid Biochem. 1985 Dec;23(6A):929-37 10964929 - J Biol Chem. 2000 Nov 17;275(46):35986-93 10679342 - Curr Opin Biotechnol. 2000 Feb;11(1):54-61 11017776 - Gen Comp Endocrinol. 2000 Sep;119(3):287-99 10821711 - J Med Chem. 2000 May 18;43(10):1986-92 9048584 - Endocrinology. 1997 Mar;138(3):863-70 11543731 - Biochem Pharmacol. 2001 Oct 1;62(7):953-61 9584619 - Trends Biochem Sci. 1998 Apr;23(4):149-50 10556875 - J Mol Recognit. 1999 Sep-Oct;12(5):279-84 9675161 - Biophys J. 1998 Aug;75(2):583-94 9658195 - Mol Pharmacol. 1998 Jul;54(1):105-12 11358690 - Chem Biol. 2001 May;8(5):427-36 9918669 - Anal Biochem. 1999 Feb 1;267(1):169-84 11431146 - J Endocrinol. 2001 Jul;170(1):137-45 11389589 - Biochemistry. 2001 Jun 12;40(23):6756-65 11437591 - Protein Expr Purif. 2001 Jul;22(2):165-73 10996572 - Pharm Sci Technolo Today. 2000 Sep 1;3(9):310-317 9927308 - Endocrinology. 1999 Feb;140(2):800-4 9751507 - Endocrinology. 1998 Oct;139(10):4252-63 10447957 - J Med Chem. 1999 Aug 12;42(16):3126-33 9685230 - J Med Chem. 1998 Jul 30;41(16):2928-31 2539536 - J Steroid Biochem. 1989 Mar;32(3):345-55 1384953 - Cancer Biochem Biophys. 1992 May;12(4):263-74 10655475 - Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1008-13 10418981 - J Steroid Biochem Mol Biol. 1999 Apr-Jun;69(1-6):51-84 Jonsson U (e_1_3_3_16_2) 1992; 2 Norris J D (e_1_3_3_7_2) 2001; 34 Schobel U (e_1_3_3_35_2) 2001; 6 e_1_3_3_17_2 e_1_3_3_19_2 e_1_3_3_18_2 e_1_3_3_39_2 e_1_3_3_13_2 e_1_3_3_36_2 e_1_3_3_12_2 e_1_3_3_37_2 e_1_3_3_15_2 e_1_3_3_34_2 e_1_3_3_14_2 e_1_3_3_32_2 e_1_3_3_33_2 e_1_3_3_11_2 e_1_3_3_30_2 e_1_3_3_10_2 e_1_3_3_31_2 e_1_3_3_40_2 Nijs M (e_1_3_3_38_2) 1992; 12 e_1_3_3_6_2 e_1_3_3_5_2 e_1_3_3_8_2 e_1_3_3_28_2 e_1_3_3_9_2 e_1_3_3_27_2 e_1_3_3_29_2 e_1_3_3_24_2 e_1_3_3_23_2 e_1_3_3_26_2 e_1_3_3_25_2 e_1_3_3_2_2 e_1_3_3_20_2 e_1_3_3_1_2 e_1_3_3_4_2 e_1_3_3_22_2 e_1_3_3_41_2 e_1_3_3_3_2 e_1_3_3_21_2 e_1_3_3_42_2 |
| References_xml | – ident: e_1_3_3_3_2 doi: 10.1016/S1461-5347(00)00288-1 – ident: e_1_3_3_25_2 doi: 10.1074/jbc.M006777200 – ident: e_1_3_3_32_2 doi: 10.1177/108705710000500204 – volume: 6 start-page: 291 year: 2001 ident: e_1_3_3_35_2 publication-title: J Biomol Screen doi: 10.1177/108705710100600503 contributor: fullname: Schobel U – ident: e_1_3_3_14_2 doi: 10.1006/prep.2001.1409 – ident: e_1_3_3_9_2 doi: 10.1021/jm980048b – volume: 12 start-page: 263 year: 1992 ident: e_1_3_3_38_2 publication-title: Cancer Biochem Biophys contributor: fullname: Nijs M – ident: e_1_3_3_37_2 doi: 10.1093/toxsci/54.1.138 – ident: e_1_3_3_12_2 doi: 10.1016/S0092-8674(00)81717-1 – ident: e_1_3_3_8_2 doi: 10.1021/jm9606352 – ident: e_1_3_3_21_2 doi: 10.1210/endo.138.3.4979 – ident: e_1_3_3_22_2 doi: 10.1006/gcen.2000.7521 – ident: e_1_3_3_11_2 doi: 10.1038/39645 – ident: e_1_3_3_26_2 doi: 10.1210/endo.139.10.6216 – ident: e_1_3_3_31_2 doi: 10.1021/bi010379h – ident: e_1_3_3_1_2 doi: 10.1006/abio.2001.5314 – ident: e_1_3_3_18_2 doi: 10.1002/(SICI)1099-1352(199909/10)12:5<279::AID-JMR473>3.0.CO;2-3 – ident: e_1_3_3_36_2 doi: 10.1210/endo.139.4.5902 – ident: e_1_3_3_13_2 doi: 10.1073/pnas.95.11.5998 – ident: e_1_3_3_28_2 doi: 10.1677/joe.0.1700137 – ident: e_1_3_3_40_2 doi: 10.1016/S1074-5521(01)00025-4 – volume: 34 start-page: 181 year: 2001 ident: e_1_3_3_7_2 publication-title: Ernst Schering Res Found Workshop contributor: fullname: Norris J D – ident: e_1_3_3_20_2 doi: 10.1016/S0006-3495(98)77549-6 – ident: e_1_3_3_33_2 doi: 10.1210/endo.140.2.6480 – ident: e_1_3_3_6_2 doi: 10.1073/pnas.97.3.1008 – ident: e_1_3_3_15_2 doi: 10.1006/abio.1998.2990 – ident: e_1_3_3_24_2 doi: 10.1124/mol.54.1.105 – ident: e_1_3_3_42_2 doi: 10.1016/0022-4731(85)90049-4 – ident: e_1_3_3_4_2 doi: 10.1016/S0958-1669(99)00054-3 – volume: 2 start-page: 291 year: 1992 ident: e_1_3_3_16_2 publication-title: Adv Biosensors contributor: fullname: Jonsson U – ident: e_1_3_3_2_2 doi: 10.1110/ps.4330102 – ident: e_1_3_3_19_2 doi: 10.1016/S0968-0004(98)01183-9 – ident: e_1_3_3_30_2 doi: 10.1074/jbc.M011651200 – ident: e_1_3_3_41_2 doi: 10.1016/0022-4731(89)90206-9 – ident: e_1_3_3_29_2 doi: 10.1016/S0960-0760(99)00065-5 – ident: e_1_3_3_5_2 doi: 10.1021/ci980140g – ident: e_1_3_3_10_2 doi: 10.1074/jbc.M009870200 – ident: e_1_3_3_39_2 doi: 10.1021/jm990078u – ident: e_1_3_3_34_2 doi: 10.1021/bi971746l – ident: e_1_3_3_17_2 doi: 10.1021/jm991174y – ident: e_1_3_3_27_2 doi: 10.1016/S0006-2952(01)00731-6 – ident: e_1_3_3_23_2 doi: 10.1289/ehp.98106551 |
| SSID | ssj0009580 |
| Score | 2.2338219 |
| Snippet | Surface plasmon resonance biosensor technology was used to directly measure the binding interactions of small molecules to the ligand-binding domain of human... |
| SourceID | pubmedcentral proquest crossref pubmed pnas jstor |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 8562 |
| SubjectTerms | Agonists Analysis Antibodies Antibodies, Monoclonal - metabolism Benzhydryl Compounds Biochemistry Biological Sciences Biosensing techniques Bisphenols Dehydroepiandrosterone - metabolism Drug interactions Estrogen Receptor alpha Estrogen Receptor beta Estrogens Kinetics Ligands Phenols - metabolism Protein isoforms Receptors Receptors, Estrogen - chemistry Receptors, Estrogen - metabolism Recombinant Proteins - chemistry Recombinant Proteins - metabolism |
| Title | Kinetic Analysis of Estrogen Receptor/Ligand Interactions |
| URI | https://www.jstor.org/stable/3059055 http://www.pnas.org/content/99/13/8562.abstract https://www.ncbi.nlm.nih.gov/pubmed/12077320 https://www.proquest.com/docview/201392177 https://search.proquest.com/docview/18413620 https://search.proquest.com/docview/71862922 https://pubmed.ncbi.nlm.nih.gov/PMC124311 |
| Volume | 99 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB3RnnpBlBZIC60PlYBDNok_EueIKqoKVMSBSr1Zju3ASq13tdn-f8aOE1pEL5xWiifZWWcm73kz8wxwhstY11mucytZH0S1da4FNZjxJbOIUFLo0Dt89a2-vOZfbsRNagobUlmlN91y4W_vFn75K9ZWru9MMdWJFd-vzhGTWFUVO7CD6Dut0GehXTm2nVB8-nLKJ6HGhhVrr4dF-M9DIgxGuVBaNg0LW30_wKSxLDFonaL5v3jn3-WTD_Do4gU8T0SSfBod3odnzr-E_ZSqA_mQ9KQ_HkD7FZkkWhGdBEjIqif4VZsVBg_BJ55boyvF7fKn9pYE_YjN2O0wHML1xecf55d52jEhN0j7t7nTrDG84r1BlLGu1Ux3dUmt7WzlTE-dlNb0ddDMkyV3opVlZ9rGtFb3lHclewW7fuXdGyB4KWSCWkqDlIvjpxVBfUybyjV4_SaD99OcqfUojKHiC-2GqTBzap7oDA7jlM5mLDS8CpHB62g4HW5bVTElkZBlQJ4YUX0qicngeLoxKmXdoGjgs7jGQudO51FMl_AORHu3ukevJKJ2TcunLRCsa9pSGtyLt_nPz0vhkkH9KABmgyDV_XgEIzhKdo8Re_S_Jx7DXtyFpqxzKt7C7nZz794hGdp2JwGKxEnMgd-AQwnS |
| link.rule.ids | 230,314,727,780,784,885,27924,27925,53791,53793 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3BbtQwEB1BOcAFtVAgtFAfkIBDNontxPYRVVQL7VYcWqk3y7EdWKnNrjbb_2ecOKFF9MIpUjxxJs6M5zmZeQb4gMtYXztuUidZE0i1TWpKatHjc-YwQsnShNrhxXk1v-Tfr8qrWBTWxbTK1tbLWXt9M2uXv_rcyvWNzcY8sezH4hhjEiuK7DE8KZlQxbhGn6h25VB4QnH-5ZSPVI2CZevWdLPw1UNiIOwJQ2kuBAubfd-JSkNiYmA7RfF_Ic-_EyjvRKSTXXgeoST5Mqi8B498-wL2orN25FNklP78EtQpYkmUIiZSkJBVQ_BWmxWaD8E5z69Rlex6-dO0jgQGic1Q79Dtw-XJ14vjeRr3TEgtAv9t6g0Tlhe8sRhnnFeGmbrKqXO1K7xtqJfS2aYKrHky575UMq-tElY501Be5-wV7LSr1r8Bgl0hFjRSWgRdHI-uDPxjxhZeYP8igY_jmOn1QI2h-1_agukwcnoa6AT2-yGdxFgoeS3LBF73guNppXTBtERIlgB5oEU3MSkmgYPxxejod52mAdHiKguVO5pa0WHCXxDT-tUtaiUxblc0f1gCw3VFFaVBvf41_3m8aC4JVPcMYBIIZN33W9CGe9LuwWbf_u-FR_B0frE402ffzk8P4Fm_J01epbQ8hJ3t5ta_Q2i0rd_3nvAbWsEMQQ |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB5RKlW9ICi0DdDiQyXoIZvEj8Q5VrQrWgriABI3y7EduhJko93l_zN2HkBVLj1FiifOxJnxfE5mPgN8wWWsqyzXsZWs9qTaOtaCGvT4lFmMUFJoXzt8dp6fXPFf1-J6DeRQCxOS9k01mzS3d5Nm9ifkVrZ3JhnyxJKLs2OMSSzLktbWySt4LRja2LBOH-l2ZVd8QnEO5pQPdI0FS9pGLyf-y4fEYBhIQ2laFMxv-P0kMnXJiZ7xFMX_hT7_TqJ8EpWmm7DRw0nyrVN7C9Zc8w62eoddkqOeVfrrNpSniCdRiuiehoTMa4K3WszRhAjOe65FVZLb2Y1uLPEsEouu5mG5A1fTH5fHJ3G_b0JsEPyvYqdZYXjGa4OxxrpSM13lKbW2spkzNXVSWlPnnjlPptyJUqaVKQtTWl1TXqXsPaw388Z9BIJdIR7UUhoEXhyPVngOMm0yV2D_RQSHw5iptqPHUOG3dsGUHzk1DnQEO2FIRzHmy16FiOBDEBxOl6XKmJIIyyIgL7Souk-MiWBveDGq972loh7V4koLlTsYW9Fp_J8Q3bj5PWolMXbnNH1ZAkN2TktKvXrhNT8-Xm8uEeTPDGAU8ITdz1vQjgNxd2e3u_974QG8ufg-Vb9_np_uwduwLU2ax1Tsw_pqce8-ITpaVZ-DIzwANX4NVA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Kinetic+analysis+of+estrogen+receptor%2Fligand+interactions&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Rich%2C+Rebecca+L&rft.au=Hoth%2C+Lise+R&rft.au=Geoghegan%2C+Kieran+F&rft.au=Brown%2C+Thomas+A&rft.date=2002-06-25&rft.issn=0027-8424&rft.volume=99&rft.issue=13&rft.spage=8562&rft.epage=8567&rft_id=info:doi/10.1073%2Fpnas.142288199&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F99%2F13.cover.gif |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F99%2F13.cover.gif |