Mitigating temporal mismatches in forensic soil microbial profiles

• Published in: Australian journal of forensic sciences Vol. 51; no. 6; pp. 685 - 694
• Main Authors: Pasternak, Zohar, Luchibia, Aineah Obed, Matan, Ofra, Dawson, Lorna, Gafny, Ron, Shpitzen, Moshe, Avraham, Shlomit, Jurkevitch, Edouard
• Format: Journal Article
• Language: English
• Published: Clovelly Taylor & Francis 02.11.2019; Copyright Agency Limited (Distributor)
• Subjects: Annual variations; Bacteria; Crime; CRIMINAL INVESTIGATION; Deoxyribonucleic acid; DNA; DNA fingerprinting; FORENSIC SCIENCE; Freeze drying; Freezing; Microorganisms; Sample preparation (Chemistry); sample storage; Seasonal variations; Soil; Soil microorganisms; Soil types; Soils; Storage; Summer; temporal mismatch; Temporal variations; TRFLP; Winter

Forensic implementation of soil bacterial DNA profiling is limited by the potential for temporal mismatch of DNA profiles, e.g. after storage or seasonal changes. We compared profiles of samples retrieved at one location over 14 years after air-drying, freeze-drying and -80 °C freezing storage. Samp...