Mechanisms of anaphylaxis in human low-affinity IgG receptor locus knock-in mice

• Published in: Journal of allergy and clinical immunology Vol. 139; no. 4; pp. 1253 - 1265.e14
• Main Authors: Gillis, Caitlin M., Jönsson, Friederike, Mancardi, David A., Tu, Naxin, Beutier, Héloïse, Van Rooijen, Nico, Macdonald, Lynn E., Murphy, Andrew J., Bruhns, Pierre
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2017; Elsevier Limited; Elsevier
• Subjects: Affinity; Allergies; Allergology; Allergy and Immunology; Anaphylaxis; Anaphylaxis - immunology; Animal models; Animals; Antigens; basophil; Disease Models, Animal; Gene Knock-In Techniques; GPI-Linked Proteins - immunology; Histamine; human FcγR; Human subjects; Humans; Hypothermia; IgG; Immunization; Immunoglobulin E; Immunoglobulin G; Immunoglobulins; Immunology; Immunotherapy; Intravenous administration; Kinases; knock-in mouse model; Leukocytes (basophilic); Leukocytes (neutrophilic); Life Sciences; macrophage; Mice; Mice, 129 Strain; Mice, Inbred C57BL; monocyte; Mortality; Myeloid cells; neutrophil; Neutrophils; Platelet-activating factor; Receptors, IgG - immunology; Rodents; Transgenic animals; SSC; histamine; BAC; IgG; macrophage; IVIG; platelet-activating factor; basophil; neutrophil; BAL; ES; ASA; Anaphylaxis; PAF; knock-in mouse model; monocyte; HA; human FcγR; NK; PAF-R; PSA; Heat aggregated; Platelet-activating factor receptor; Bronchoalveolar lavage; Active systemic anaphylaxis; Intravenous immunoglobulin; Embryonic stem; Side scatter; Natural killer; Passive systemic anaphylaxis; Bacterial artificial chromosome

Anaphylaxis can proceed through distinct IgE- or IgG-dependent pathways, which have been investigated in various mouse models. We developed a novel mouse strain in which the human low-affinity IgG receptor locus, comprising both activating (hFcγRIIA, hFcγRIIIA, and hFcγRIIIB) and inhibitory (hFcγRII...