Learn from the SARS-CoV-2 nucleic acid test to increase the experience of dealing with the “disease X

• Published in: BMC infectious diseases Vol. 25; no. 1; pp. 593 - 9
• Main Authors: Yang, Xin, Li, Jinming
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24.04.2025; BioMed Central; BMC
• Subjects: Acids; Chemical tests and reagents; Chi-square test; China; Coronaviruses; COVID-19; COVID-19 - diagnosis; COVID-19 Nucleic Acid Testing - methods; Cycle threshold; Data collection; Disease control; Disease prevention; Diseases; Epidemics; False positive; False Positive Reactions; Gene amplification; Genes; Health aspects; Humans; Israel; Laboratories; Medical screening; Nucleic acids; Pandemics; Polymerase chain reaction; Quality control; Reagents; Respiratory diseases; Retest; RT‒PCR; SARS-CoV-2 - genetics; SARS-CoV-2 - isolation & purification; SARS‒CoV‒2; Sensitivity and Specificity; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Synthesis; Testing; Tubes; Viral diseases; China; Israel; Shanghai China; COVID‒19; False positive; Retest; Cycle threshold; RT‒PCR; Disease X; SARS‒CoV‒2

RT‒PCR is crucial for screening for epidemic diseases such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, false positives further plague epidemic prevention and control. This study conducted a stratified study on the initial screening Ct values and false positive ratios, p...