Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 109; no. 1; pp. 22 - 27
• Main Authors: Ji, Na, Sato, Takashi R, Betzig, Eric
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 03.01.2012; National Acad Sciences
• Subjects: Adaptive optics; Animals; Biological Sciences; Brain; Calcium; Calcium - metabolism; Cerebral Cortex - anatomy & histology; cortex; Fluorescence; image analysis; Imaging; Imaging, Three-Dimensional - methods; Medical imaging; Mice; Microscopy; Neuroimaging; Neurons; Optics; Optics and Photonics; Physical Sciences; Rodents; Skull; Wave excitation

The signal and resolution during in vivo imaging of the mouse brain is limited by sample-induced optical aberrations. We find that, although the optical aberrations can vary across the sample and increase in magnitude with depth, they remain stable for hours. As a result, two-photon adaptive optics...