Defining multistep cell fate decision pathways during pancreatic development at single‐cell resolution

• Published in: The EMBO journal Vol. 38; no. 8
• Main Authors: Yu, Xin‐Xin, Qiu, Wei‐Lin, Yang, Liu, Zhang, Yu, He, Mao‐Yang, Li, Lin‐Chen, Xu, Cheng‐Ran
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.04.2019; Blackwell Publishing Ltd; John Wiley and Sons Inc
• Subjects: Animals; Cell Differentiation; Cell fate; cell fate choice; Cell Lineage; Differentiation; EMBO11; EMBO22; EMBO43; Embryos; fate map; Female; Fetuses; Gene expression; Gene Expression Regulation, Developmental; Gene sequencing; Genetic modification; MAP kinase; MAPK/ERK; Metabolic pathways; Mice; Organogenesis; Organs; Pancreas; Pancreas - cytology; Pancreas - metabolism; pancreatic lineage; Populations; Regulatory mechanisms (biology); Resource; Ribonucleic acid; RNA; Single-Cell Analysis - methods; single‐cell RNA‐seq; Stem cell transplantation; Stem cells; Trajectories; Zebrafish; cell fate choice; single‐cell RNA‐seq; MAPK/ERK; pancreatic lineage; fate map

The generation of terminally differentiated cell lineages during organogenesis requires multiple, coordinated cell fate choice steps. However, this process has not been clearly delineated, especially in complex solid organs such as the pancreas. Here, we performed single‐cell RNA‐sequencing in pancr...