Metabolomics‐driven quantitative analysis of ammonia assimilation in E. coli

• Published in: Molecular systems biology Vol. 5; no. 1; pp. 302 - n/a
• Main Authors: Yuan, Jie, Doucette, Christopher D, Fowler, William U, Feng, Xiao‐Jiang, Piazza, Matthew, Rabitz, Herschel A, Wingreen, Ned S, Rabinowitz, Joshua D
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 2009; John Wiley & Sons, Ltd; EMBO Press; Nature Publishing Group; Springer Nature
• Subjects: active‐site competition; Ammonia; Ammonia - metabolism; Ammonium; Assimilation; Biological assimilation; Biomass; Catalytic Domain; Chromatography, High Pressure Liquid; Competition; Dehydrogenases; Differential equations; Dynamic response; E coli; EMBO10; EMBO26; Enzymes; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; flux regulation; Fluxes; Genetic algorithms; Genetic Techniques; Glutamate-ammonia ligase; Glutamine; Glutamine - metabolism; Growth rate; Homeostasis; HPLC‐MS; Intermediates; Ketoglutaric acid; Ketoglutaric Acids - metabolism; Mass Spectrometry - methods; metabolic dynamics; Metabolism; Metabolites; Metabolome; Metabolomics; Metabolomics - methods; Models, Genetic; Nitrogen; Nitrogen - metabolism; Parameter identification; Perturbation; predictive model; Proteins; Quantitative analysis; Quaternary Ammonium Compounds; Substrates; Systems Biology - methods; active‐site competition; predictive model; flux regulation; HPLC‐MS; metabolic dynamics
• ISSN: 1744-4292; 1744-4292
• DOI: 10.1038/msb.2009.60

Despite extensive study of individual enzymes and their organization into pathways, the means by which enzyme networks control metabolite concentrations and fluxes in cells remains incompletely understood. Here, we examine the integrated regulation of central nitrogen metabolism in Escherichia coli...