单宁酶处理提高茶梗儿茶素含量及茶梗提取液生物活性

为综合利用茶叶加工副产物茶叶梗,试验设计利用固态发酵得到的单宁酶处理茶梗,比较不同处理条件下的茶梗提取液中儿茶素组成的差异及还原力、DPPH和OH自由基清除率、胰α-淀粉酶和胰脂肪酶抑制活性变化,探讨单宁酶处理提高茶梗提取物中儿茶素含量和生物活性可行性。研究发现：在50℃条件下,利用2U/mL单宁酶溶液作用于茶梗粉末60min,茶梗提取液中酯型儿茶素（EGCG、ECG、GCG）基本被水解生成非酯型儿茶素（EGC、EC、GC）和没食子酸（GA）,从而减少单宁-蛋白质聚合物和茶乳的形成量;此外,经单宁酶处理的茶梗提取液抗氧化活性与对照比较明显增强,表现为OH和DPPH自由基清除率IC50分别降低了...

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Bibliographic Details
Published in农业工程学报 Vol. 29; no. 13; pp. 277 - 285
Main Author 黄玉凤 肖安风 倪辉 蔡慧农
Format Journal Article
LanguageChinese
Published 福建省高校食品微生物与酶工程技术研究中心，厦门 361021 2013
集美大学生物工程学院,厦门,361021%集美大学生物工程学院，厦门 361021
厦门市食品与生物工程技术研究中心，厦门 361021
福建省食品微生物与酶工程重点实验室，厦门 361021
Subjects
儿茶素
提取
生物活性
茶梗
转化
酶
茶梗
catechins
bioactivity
tea stalk
酶
儿茶素
enzymes
提取
转化
extraction
transformation
生物活性
Online AccessGet full text
ISSN1002-6819
DOI10.3969/j.issn.1002-6819.2013.13.035

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Summary:为综合利用茶叶加工副产物茶叶梗,试验设计利用固态发酵得到的单宁酶处理茶梗,比较不同处理条件下的茶梗提取液中儿茶素组成的差异及还原力、DPPH和OH自由基清除率、胰α-淀粉酶和胰脂肪酶抑制活性变化,探讨单宁酶处理提高茶梗提取物中儿茶素含量和生物活性可行性。研究发现：在50℃条件下,利用2U/mL单宁酶溶液作用于茶梗粉末60min,茶梗提取液中酯型儿茶素（EGCG、ECG、GCG）基本被水解生成非酯型儿茶素（EGC、EC、GC）和没食子酸（GA）,从而减少单宁-蛋白质聚合物和茶乳的形成量;此外,经单宁酶处理的茶梗提取液抗氧化活性与对照比较明显增强,表现为OH和DPPH自由基清除率IC50分别降低了74%和26%;酶解茶梗提取液质量浓度为5000mg/L时,胰α-淀粉酶和胰脂肪酶抑制率分别提高了89%和107%。研究结果表明单宁酶可高效水解茶梗提取液中酯型儿茶素,提高茶梗提取液的抗氧化活性以及体外抑制胰α-淀粉酶和胰脂肪酶活性。
Bibliography:11-2047/S
Huang Yufeng, Xiao Anfeng, Ni Hui, Cai Huinong （1. College of Bioengineering, Jimei University, Xiamen 361021, China; 2. Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province, Xiamen 361021, China; 3. Research Center of Food Microbiology and Enzyme Engineering Technology of Fujian Province, Xiamen 361021, China; 4. Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China）
Tea stalk, also known as tea stem, a residue obtained from the process of initial tea selection, is a very abundant and low-cost agricultural byproduct which is plentifully available in southern China, and its use has not been exploited and standardized. In this study, in order to use the agricultural byproduct, tea stalk, thoroughly, tea stalk infusion was prepared with the help of tannase preparation from Aspergillus niger JMU-TS528 by the solidstate fermentation of tea stalks. Then, the property and effectiveness of a tannase-aided treatment on the contents of catechins and bioactivity cha
ISSN:1002-6819
DOI:10.3969/j.issn.1002-6819.2013.13.035