XPB, a subunit of TFIIH, is a target of the natural product triptolide

• Published in: Nature chemical biology Vol. 7; no. 3; pp. 182 - 188
• Main Authors: Liu, Jun O, Titov, Denis V, Gilman, Benjamin, He, Qing-Li, Bhat, Shridhar, Low, Woon-Kai, Dang, Yongjun, Smeaton, Michael, Demain, Arnold L, Miller, Paul S, Kugel, Jennifer F, Goodrich, James A
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2011; Nature Publishing Group
• Subjects: 631/154/555; 631/92/349; 631/92/609; Adenosine triphosphatase; Adenosine Triphosphatases - chemistry; Adenosine Triphosphatases - metabolism; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Biochemical Engineering; Biochemistry; Biological Products - chemistry; Biological Products - isolation & purification; Biological Products - pharmacology; Bioorganic Chemistry; Cell Biology; Cell Survival - drug effects; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chinese medicine; Deoxyribonucleic acid; Diterpenes - chemistry; Diterpenes - isolation & purification; Diterpenes - pharmacology; DNA; DNA Helicases - chemistry; DNA Helicases - metabolism; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - metabolism; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Epoxy Compounds - chemistry; Epoxy Compounds - isolation & purification; Epoxy Compounds - pharmacology; Eukaryotes; Flowers & plants; HeLa Cells; Humans; Medicinal plants; Phenanthrenes - chemistry; Phenanthrenes - isolation & purification; Phenanthrenes - pharmacology; Protein Binding; Protein Subunits - chemistry; Protein Subunits - metabolism; RNA Polymerase II - genetics; RNA Polymerase II - metabolism; Transcription Factor TFIIH - chemistry; Transcription Factor TFIIH - metabolism

Triptolide is a bioactive natural product isolated from plants used in traditional Chinese medicine. A target identification approach shows that triptolide modulates RNA transcription and nucleotide excision repair by covalent binding to the XPB subunit of the transcription factor TFIIH. Triptolide...