Bioengineered 3D platform to explore cell–ECM interactions and drug resistance of epithelial ovarian cancer cells

• Published in: Biomaterials Vol. 31; no. 32; pp. 8494 - 8506
• Main Authors: Loessner, Daniela, Stok, Kathryn S, Lutolf, Matthias P, Hutmacher, Dietmar W, Clements, Judith A, Rizzi, Simone C
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.11.2010
• Subjects: Advanced Basic Science; Antineoplastic Agents, Phytogenic - pharmacology; Bioengineering; Biomimetic material; Biomimetic Materials - chemistry; Biomimetic Materials - metabolism; Cell encapsulation; Cell Line, Tumor; Cell morphology; Cell Survival - drug effects; Cell viability; Dentistry; Drug Resistance, Neoplasm; ECM; Epithelial Cells - cytology; Epithelial Cells - metabolism; Female; Humans; Hydrogel; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism; Integrins - metabolism; Mechanical Phenomena; Oligopeptides - chemistry; Oligopeptides - metabolism; Ovarian Neoplasms - drug therapy; Ovarian Neoplasms - metabolism; Paclitaxel - pharmacology; Polyethylene Glycols - chemistry; Polyethylene Glycols - metabolism; Spheroids, Cellular - cytology; Cell viability; Hydrogel; Biomimetic material; Cell encapsulation; ECM; Cell morphology

Abstract The behaviour of cells cultured within three-dimensional (3D) structures rather than onto two-dimensional (2D) culture plastic more closely reflects their in vivo responses. Consequently, 3D culture systems are becoming crucial scientific tools in cancer cell research. We used a novel 3D cu...