Effect of kinematic viscosity on the staining performance of caries detector dyes

• Published in: Dental Materials Journal Vol. 38; no. 1; pp. 120 - 126
• Main Authors: KOBAYASHI, Mikihiro, INAGAKI, Rina, ICHIKAWA, Kotona, NIIZUMA, Yuiko, MORISAKI, Hirobumi, KUWATA, Hirotaka, MANABE, Atsufumi, MIYAZAKI, Takashi
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japanese Society for Dental Materials and Devices 28.01.2019; Japanese Society for Dental Materials and Devices; Japan Science and Technology Agency
• Subjects: Bicuspid; Calcification; Caries detection; Coloring Agents - chemistry; Debris; Dental caries; Dental Caries - diagnosis; Dentin; Dyes; Humans; Immunohistochemistry; In Vitro Techniques; Kinematic viscosity; Kinematics; Microscopy, Electron, Scanning; Molar; Molecular weight; Propylene Glycols - chemistry; Rhodamines - chemistry; Scanning electron microscopy; Sensors; Smear Layer; Solvents; Staining; Staining performance; Surface Properties; Tooth Discoloration - chemically induced; Tubules; Viscosity; Immunohistochemistry; Caries detection; Staining performance; Kinematic viscosity
• ISSN: 0287-4547; 1881-1361
• DOI: 10.4012/dmj.2017-083

The relationship between performance of caries detector dyes and solvent molecular weight has been reported, but viscosity has not yet been investigated. Therefore, using extracted human teeth, we compared 6 experimental caries detector dyes with different viscosities to clarify the relationship between dye viscosity and staining performance in sclerotic dentin. Carious dentin started showing dentin staining when stained with caries detector dyes with kinematic viscosity≥263 mm2/s, showed no immunoreactivity for Streptococcus mutans on immunohistochemistry, and contained dentinal tubules free of debris on scanning electron microscopy. Samples stained using a caries detector dye with kinematic viscosity of 332 mm2/s contained calcification product-like debris in the dentinal tubules. These findings suggest that the caries detector dye has a threshold kinematic viscosity between 263 and 332 mm2/s at which sclerotic dentin and tissues beyond are not stained. The caries detector dye appears to control tooth dyeability based on solvent viscosity.