Platinum Nanoparticles Enhance Exosome Release in Human Lung Epithelial Adenocarcinoma Cancer Cells (A549): Oxidative Stress and the Ceramide Pathway are Key Players

• Published in: International journal of nanomedicine Vol. 16; pp. 515 - 538
• Main Authors: Gurunathan, Sangiliyandi, Kang, Min-Hee, Jeyaraj, Muniyandi, Kim, Jin-Hoi
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2021; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: A549 Cells; Acetylcholinesterase - metabolism; acetylcholinesterase activity; Acetylcysteine; Acetylcysteine - pharmacology; Adenocarcinoma; Adenocarcinoma of Lung - genetics; Adenocarcinoma of Lung - metabolism; Adenocarcinoma of Lung - pathology; Analysis; Aniline Compounds - pharmacology; Apoptosis; Benzylidene Compounds - pharmacology; Biomarkers; Biosynthesis; Cancer; Cancer cells; Cancer therapies; Cell growth; Cell Proliferation - drug effects; Cell Survival - drug effects; Ceramides - metabolism; Cytotoxicity; Enzyme Activation - drug effects; Enzyme-linked immunosorbent assay; exosome; Exosomes - metabolism; Exosomes - ultrastructure; Gene Expression Regulation, Neoplastic - drug effects; Humans; Hypoxia; Kinases; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Lutein - pharmacology; Melanoma; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Microscopy; Nanoparticles; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; neutral sphingomyelinase activity; Oncology, Experimental; Original Research; Oxidative stress; Oxidative Stress - drug effects; Particle Size; Physiological aspects; Platinum - pharmacology; platinum nanoparticle; Prevention; Product introduction; Proteins; RNA, Messenger - genetics; RNA, Messenger - metabolism; Serum; Sphingomyelin Phosphodiesterase - metabolism; Static Electricity; United States > US; neutral sphingomyelinase activity; platinum nanoparticle; acetylcholinesterase activity; exosome; cytotoxicity; oxidative stress

Several studies have demonstrated various molecular mechanisms involved in the biogenesis and release of exosomes. However, how external stimuli, such as platinum nanoparticles (PtNPs), induces the biogenesis and release of exosomes remains unclear. To address this, PtNPs were synthesized using lute...