Histopathological and immunohistochemical examination of the brains of rabid dogs in the Philippines
Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its detailed neuropathology. The present study analyzed the neuropathology in the cerebrum, hippocampus, thalamus, and brainstem of 70 rabid dogs with c...
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| Published in | Journal of Veterinary Medical Science Vol. 86; no. 12; pp. 24-0249 - 1251 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Japan
JAPANESE SOCIETY OF VETERINARY SCIENCE
2024
Japan Science and Technology Agency The Japanese Society of Veterinary Science |
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| Abstract | Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its detailed neuropathology. The present study analyzed the neuropathology in the cerebrum, hippocampus, thalamus, and brainstem of 70 rabid dogs with confirmed rabies infection in the Philippines. Histopathologically, inclusion bodies (Negri bodies) were detected in the hippocampus (87.14%), cerebrum (70%), and thalamus (2.86%) of the dogs. The inclusion bodies in the cytoplasm of the hippocampal and cerebral cortical pyramidal cells were large and round to oval in shape. Whereas the inclusion bodies in the neurons of the thalamus and brainstem were small, fine, and granular. In contrast to the high prevalence of inclusion bodies in the hippocampus and cerebrum, perivascular cuffing and glial nodules were more prominent in the thalamus and brainstem. Immunohistochemically using the anti-phosphoprotein (anti-P), the sensitivity of viral antigen detection was 100% in the hippocampus, thalamus, and brainstem and 97.14% in the cerebrum. Our findings confirmed that observing the inclusion bodies in the hippocampus and cerebral cortex by histopathology could facilitate rabies diagnosis in the dogs in the Philippines, and furthermore, using immunohistochemistry on the brainstem could also be useful to detect rabies virus antigens with high sensitivity. |
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| AbstractList | Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its detailed neuropathology. The present study analyzed the neuropathology in the cerebrum, hippocampus, thalamus, and brainstem of 70 rabid dogs with confirmed rabies infection in the Philippines. Histopathologically, inclusion bodies (Negri bodies) were detected in the hippocampus (87.14%), cerebrum (70%), and thalamus (2.86%) of the dogs. The inclusion bodies in the cytoplasm of the hippocampal and cerebral cortical pyramidal cells were large and round to oval in shape. Whereas the inclusion bodies in the neurons of the thalamus and brainstem were small, fine, and granular. In contrast to the high prevalence of inclusion bodies in the hippocampus and cerebrum, perivascular cuffing and glial nodules were more prominent in the thalamus and brainstem. Immunohistochemically using the anti-phosphoprotein (anti-P), the sensitivity of viral antigen detection was 100% in the hippocampus, thalamus, and brainstem and 97.14% in the cerebrum. Our findings confirmed that observing the inclusion bodies in the hippocampus and cerebral cortex by histopathology could facilitate rabies diagnosis in the dogs in the Philippines, and furthermore, using immunohistochemistry on the brainstem could also be useful to detect rabies virus antigens with high sensitivity. Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its detailed neuropathology. The present study analyzed the neuropathology in the cerebrum, hippocampus, thalamus, and brainstem of 70 rabid dogs with confirmed rabies infection in the Philippines. Histopathologically, inclusion bodies (Negri bodies) were detected in the hippocampus (87.14%), cerebrum (70%), and thalamus (2.86%) of the dogs. The inclusion bodies in the cytoplasm of the hippocampal and cerebral cortical pyramidal cells were large and round to oval in shape. Whereas the inclusion bodies in the neurons of the thalamus and brainstem were small, fine, and granular. In contrast to the high prevalence of inclusion bodies in the hippocampus and cerebrum, perivascular cuffing and glial nodules were more prominent in the thalamus and brainstem. Immunohistochemically using the anti-phosphoprotein (anti-P), the sensitivity of viral antigen detection was 100% in the hippocampus, thalamus, and brainstem and 97.14% in the cerebrum. Our findings confirmed that observing the inclusion bodies in the hippocampus and cerebral cortex by histopathology could facilitate rabies diagnosis in the dogs in the Philippines, and furthermore, using immunohistochemistry on the brainstem could also be useful to detect rabies virus antigens with high sensitivity.Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its detailed neuropathology. The present study analyzed the neuropathology in the cerebrum, hippocampus, thalamus, and brainstem of 70 rabid dogs with confirmed rabies infection in the Philippines. Histopathologically, inclusion bodies (Negri bodies) were detected in the hippocampus (87.14%), cerebrum (70%), and thalamus (2.86%) of the dogs. The inclusion bodies in the cytoplasm of the hippocampal and cerebral cortical pyramidal cells were large and round to oval in shape. Whereas the inclusion bodies in the neurons of the thalamus and brainstem were small, fine, and granular. In contrast to the high prevalence of inclusion bodies in the hippocampus and cerebrum, perivascular cuffing and glial nodules were more prominent in the thalamus and brainstem. Immunohistochemically using the anti-phosphoprotein (anti-P), the sensitivity of viral antigen detection was 100% in the hippocampus, thalamus, and brainstem and 97.14% in the cerebrum. Our findings confirmed that observing the inclusion bodies in the hippocampus and cerebral cortex by histopathology could facilitate rabies diagnosis in the dogs in the Philippines, and furthermore, using immunohistochemistry on the brainstem could also be useful to detect rabies virus antigens with high sensitivity. Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its detailed neuropathology. The present study analyzed the neuropathology in the cerebrum, hippocampus, thalamus, and brainstem of 70 rabid dogs with confirmed rabies infection in the Philippines. Histopathologically, inclusion bodies (Negri bodies) were detected in the hippocampus (87.14%), cerebrum (70%), and thalamus (2.86%) of the dogs. The inclusion bodies in the cytoplasm of the hippocampal and cerebral cortical pyramidal cells were large and round to oval in shape. Whereas the inclusion bodies in the neurons of the thalamus and brainstem were small, fine, and granular. In contrast to the high prevalence of inclusion bodies in the hippocampus and cerebrum, perivascular cuffing and glial nodules were more prominent in the thalamus and brainstem. Immunohistochemically using the anti-phosphoprotein (anti-P), the sensitivity of viral antigen detection was 100% in the hippocampus, thalamus, and brainstem and 97.14% in the cerebrum. Our findings confirmed that observing the inclusion bodies in the hippocampus and cerebral cortex by histopathology could facilitate rabies diagnosis in the dogs in the Philippines, and furthermore, using immunohistochemistry on the brainstem could also be useful to detect rabies virus antigens with high sensitivity. |
| ArticleNumber | 24-0249 |
| Author | NACION, Leilanie B. PARK, Chun-Ho IAMOHBHARS, Nuttipa KIMITSUKI, Kazunori MANALO, Daria Llenarresas ESPINO, Mark Joseph M. SHIWA-SUDO, Nozomi CABIC, Alpha Grace B MARKBORDEE, Boonkanit INOUE, Satoshi |
| Author_xml | – sequence: 1 fullname: MARKBORDEE, Boonkanit organization: Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University – sequence: 2 fullname: CABIC, Alpha Grace B organization: Research Institute for Tropical Medicine, Department of Health, Republic of the Philippines – sequence: 3 fullname: IAMOHBHARS, Nuttipa organization: Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University – sequence: 4 fullname: SHIWA-SUDO, Nozomi organization: Department of Pathology, National Institute of Infectious Diseases – sequence: 5 fullname: KIMITSUKI, Kazunori organization: Department of Microbiology, Faculty of Medicine, Oita University – sequence: 6 fullname: ESPINO, Mark Joseph M. organization: Research Institute for Tropical Medicine, Department of Health, Republic of the Philippines – sequence: 7 fullname: NACION, Leilanie B. organization: Research Institute for Tropical Medicine, Department of Health, Republic of the Philippines – sequence: 8 fullname: MANALO, Daria Llenarresas organization: Research Institute for Tropical Medicine, Department of Health, Republic of the Philippines – sequence: 9 fullname: INOUE, Satoshi organization: Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University – sequence: 10 fullname: PARK, Chun-Ho organization: Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University |
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| Snippet | Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its... Dogs are the primary transmitters of the rabies virus in the Philippines; however, to the best of our knowledge, no published studies have examined its... |
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| SubjectTerms | Animals Antigens, Viral - analysis Bats brain Brain - pathology Brain - virology Brain stem Cerebral cortex Cerebrum Cytoplasm Dog Diseases - pathology Dog Diseases - virology Dogs Female Hippocampus Hippocampus - pathology histopathology Immunohistochemistry Immunohistochemistry - veterinary Inclusion bodies Inclusion Bodies, Viral Lyssavirus Male Negri bodies Neuronal-glial interactions Neuropathology Pathology Philippines Pyramidal cells rabid dog Rabies Rabies - pathology Rabies - veterinary Rabies - virology Rabies virus - isolation & purification Thalamus |
| Title | Histopathological and immunohistochemical examination of the brains of rabid dogs in the Philippines |
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