Droplet digital PCR assay provides intrahepatic HBV cccDNA quantification tool for clinical application

• Published in: Scientific reports Vol. 12; no. 1; pp. 2133 - 14
• Main Authors: Hayashi, Sanae, Isogawa, Masanori, Kawashima, Keigo, Ito, Kyoko, Chuaypen, Natthaya, Morine, Yuji, Shimada, Mitsuo, Higashi-Kuwata, Nobuyo, Watanabe, Takehisa, Tangkijvanich, Pisit, Mitsuya, Hiroaki, Tanaka, Yasuhito
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.02.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 692/4020/4021; 692/699/1503/234/2513/1549; Animals; Antigens; Biopsy; Carcinoma, Hepatocellular - virology; Circular DNA; DNA, Circular - analysis; DNA, Viral - analysis; Hepatitis B; Hepatitis B - drug therapy; Hepatitis B - virology; Hepatitis B e antigen; Hepatitis B surface antigen; Hepatitis B virus - genetics; Hepatocellular carcinoma; Humanities and Social Sciences; Humans; Interferon; Interferon-alpha - therapeutic use; Liver; Liver - virology; Liver cancer; Mice, Transgenic; multidisciplinary; Patients; Polyethylene Glycols - therapeutic use; Polymerase chain reaction; Polymerase Chain Reaction - methods; Quantitation; Recombinant Proteins - therapeutic use; Repopulation; Science; Science (multidisciplinary); Tumors

The persistence of covalently closed circular DNA (cccDNA) poses a major obstacle to curing chronic hepatitis B (CHB). Here, we used droplet digital PCR (ddPCR) for cccDNA quantitation. The cccDNA-specific ddPCR showed high accuracy with the dynamic range of cccDNA detection from 10 1 to 10 5 copies...