Changes in endoplasmic reticulum stress proteins and aldolase A in cells exposed to dopamine

• Published in: Journal of neurochemistry Vol. 106; no. 1; pp. 333 - 346
• Main Authors: Dukes, April A, Van Laar, Victor S, Cascio, Michael, Hastings, Teresa G
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.07.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: 2D difference in-gel electrophoresis; Animals; Biochemistry; Biological and medical sciences; Calreticulin - drug effects; Calreticulin - metabolism; Cellular biology; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; dopamine; Dopamine - metabolism; Dopamine - pharmacology; Down-Regulation - drug effects; Down-Regulation - physiology; Electrophoresis, Gel, Two-Dimensional; Endoplasmic Reticulum - drug effects; Endoplasmic Reticulum - metabolism; endoplasmic reticulum stress; Fluorescent Dyes; Fructose-Bisphosphate Aldolase - drug effects; Fructose-Bisphosphate Aldolase - metabolism; Heat-Shock Proteins - drug effects; Heat-Shock Proteins - metabolism; HSP70 Heat-Shock Proteins - drug effects; HSP70 Heat-Shock Proteins - metabolism; Mass Spectrometry; Medical sciences; Membrane Glycoproteins - drug effects; Membrane Glycoproteins - metabolism; Membrane Proteins - drug effects; Membrane Proteins - metabolism; mitochondria; Molecular Chaperones - drug effects; Molecular Chaperones - metabolism; Nervous system (semeiology, syndromes); Nervous system as a whole; Neurology; Neurons - drug effects; Neurons - metabolism; Oxidation; Oxidative Stress - drug effects; Oxidative Stress - physiology; Parkinson Disease - metabolism; Parkinson Disease - physiopathology; Parkinson's disease; PC12; PC12 Cells; Protein Disulfide-Isomerases - drug effects; Protein Disulfide-Isomerases - metabolism; Protein Folding; Proteins - drug effects; Proteins - metabolism; proteomics; Rats; Up-Regulation - drug effects; Up-Regulation - physiology; Modification; Oxidative stress; Toxicity; Parkinson disease; 2D difference in-gel electrophoresis; endoplasmic reticulum stress; Unfolded protein response; Aggregation; Mitochondria; Degenerative disease; Oxidation; Nervous system diseases; Dopamine; proteomics; Catecholamine; Metabolism; In vitro; Protein; Cerebral disorder; PC12; Dysfunction; Central nervous system disease; Neurotransmitter; Endoplasmic reticulum; Extrapyramidal syndrome

In Parkinson's disease, oxidative stress is implicated in protein misfolding and aggregation, which may activate the unfolded protein response by the endoplasmic reticulum (ER). Dopamine (DA) can initiate oxidative stress via H₂O₂ formation by DA metabolism and by oxidation into DA quinone. We...